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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(12): 308-322
DOI: https://doi.org/10.20546/ijcmas.2020.912.041


Methylation Pattern of Repetitive Elements in Correlation with Genome Wide Global DNA Methylation in the Prognosis of Paediatric Tuberculosis
Gayathri Pandurangan, NoyalMariya Joseph and Mahadevan Subramaniyan*
1Department of Pediatrics,
2Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India
*Corresponding author
Abstract:

Epigenetic modulation plays a vital role in regulating the transcriptional machinery of human genome in response to infection. Differential Methylation pattern of the genome especially the retro transposon repetitive elements (RE) which constitutes more than 45% of the genome had been studied to trace the impact of RE methylation in the disease severity. The correlation of the levels of genome wide DNA methylation and the methylation levels of LINE1 (Long interspersed transposable elements), SINE- Alu (Short interspersed transposable elements) repetitive elements (RE) showed the influence of repetitive elements in the prognosis of Mycobacterium tuberculosis among pediatric population. In comparison with healthy controls in this study we have investigated the association of methylation status altering the transcriptome involved in eliciting immune response. Investigating the levels of methylation of the repetitive elements will be an index to analyze the genome wide methylation which implicates the severity of the disease. Genome wide Methylation profiling could also be a potential biomarker to correlate with the prognosis of tuberculosis. The global methylation status of children infected with TB was found to be significantly differentiated when compared with healthy children. The methylation pattern of the repetitive elements LINE1&SINE (Alu) were also found to be significantly differentiated with that of controls. Significant genome wide hypomethylation was observed in children infected with TB (9% (IQR, 7%-10%) in comparison with healthy children (22 % (IQR, 18%-24%) (P<0.001). The repetitive elements LINE1 and SINE (Alu) in accordance with global methylation of the genome were found to be hypomethylated in children infected with TB. The percentage of LINE1 Methylation was 22% (IQR, 20%-26%) in cases and 67% (IQR 62-70%) in controls(P<0.0001) respectively. Similarly, remarkable difference in the levels of SINE(Alu) Methylation was found between cases (27% (IQR 24-30%) and controls 65% (IQR 58-68%) (P <0.0001)


Keywords: Genome wide DNA Methylation, 5- MC Quantification, Methylation Specific -PCR, Paediatric Tuberculosis, SINE(Alu), LINE1 methylation& Hypomethylation of REs.
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How to cite this article:

Gayathri Pandurangan, NoyalMariya Joseph and Mahadevan Subramaniyan. 2020. Methylation Pattern of Repetitive Elements in Correlation with Genome Wide Global DNA Methylation in the Prognosis of Paediatric Tuberculosis.Int.J.Curr.Microbiol.App.Sci. 9(12): 308-322. doi: https://doi.org/10.20546/ijcmas.2020.912.041