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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(12): 137-143
DOI: https://doi.org/10.20546/ijcmas.2020.912.018


Genetic Diversity Analysis of Indian Mustard (Brassica spp.) Germplasm Lines using SSR Molecular Markers
Ravi Baghel1, A. K. Sharma1, Sushma Tiwari1, M. K. Tripathi1* and Niraj Tripathi2
1Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior-474002, India
2Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur-482004, India
*Corresponding author
Abstract:

Molecular categorization of 48 mustard (Brassica spp.) genotypes by employing 20 SSR markers divulged that eight primers viz., BRMS-240, BRMS-324, SR-7223, SR-9222, SR-94102, OI10-C05, SSRNa10-DO9 and SSRNa 10-D11 illustrated good technical resolution and out of these eight seven furnished adequate variations among diverse genotypes. In total 50% polymorphism was detected. Major group restrained 48 genotypes that divided into three major groups, first main group ‘I’ contained 17 genotypes, second chief group ‘II’ hold 24 genotypes and third core group ‘III’ included 7 genotypes. Polymorphic information content (PIC) was estimated for each of the 7 SSR markers. The highest PIC value (0.6851) was documented for the molecular marker OI10-CO 5. It had 8 alleles among the 48 genotypes; whereas, the lowest PIC (0.4038) was examined for the molecular marker SR-7223.


Keywords: Indian mustard, Cluster analysis, Diversity analysis, SSR marker
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How to cite this article:

Ravi Baghel, A. K. Sharma, M. K. Tripathi and Niraj Tripathi. 2020. Genetic Diversity Analysis of Indian Mustard (Brassica spp.) Germplasm Lines using SSR Molecular Markers.Int.J.Curr.Microbiol.App.Sci. 9(12): 137-143. doi: https://doi.org/10.20546/ijcmas.2020.912.018