National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Klebsiella is an opportunistic bacterial pathogen of man and animals known for presence of diversity of Anti Microbial Resistance (AMR). The present study was undertaken to isolate and study the anti-microbial resistance in Klebsiella isolates from cattle and buffaloes. Sixty (60) samples, comprising of 33 mastitic milk and 27 diarrhoeic faecal samples were collected aseptically from cattle and buffaloes. From these 60 samples, 11 (18.33%) Klebsiella species were isolated and identified on the basis of cultural, morphological and biochemical characteristics. All these isolates were later confirmed as Klebsiella pnuemoniae by PCR assay using species specific primers for Klebsiella pneumoniae. Out of these 11 isolates, 8 belonged to diarrhoeic faecal and 3 belonged to mastitic milk samples. The anti-microbial sensitivity test revealed that all Klebsiella pneumoniae isolates were resistant to penicillin, amoxicillin, cefuroxime, and ampicillin/sulbactum (100.0%), followed by streptomycin (83.3%), amikacin (75.0%), cefalexin (58.3%), ceftriaxone (41.6%), gentamicin (25%), imipenum, co-trimoxazole (Sulph-trimethoprim), and ciprofloxacin (16.6% each), and norfloxacin and ofloxacin (8.3% each). However, no resistance was observed against tetracycline. Multiple Drug Resistance (MDR) was recorded in all the isolates.
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