National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Present study was aimed to understand the bacterial community composition, diversity, richness, carrying capacity and population dynamics present on soybean leaf in response to Panchagavya sprays using DGGE (Denaturing Gradient Gel Electrophoresis) and molecular fingerprinting technique. The seeds of soybean variety JS-335 were collected, surface sterilized and used in a pot culture study. In-vivo pot experiment was carried out in four sets. First three sets were sprayed on 15th day after sowing (DAS), 30th DAS and at both 15th and 30th DAS respectively and the fourth set was kept as control without spraying throughout the experiment period. The metagenomic DNA was obtained in the range of 800 to 1214 ng /μl. The Shannon diversity, Range Weighted Richness and Pielou’s evenness values clearly indicated that spraying of Panchagavya twice at 15th and 30th DAS increased bacterial diversity and microbial carrying capacity and even distribution of species in phyllosphere as compared to single time spraying and control samples without spraying. Pareto Lorenz (PL) curve values obtained revealed that more than 80 % of bacterial diversity was represented by a specialized community present in phyllosphere. The bacterial dynamics in sprayed samples were higher than their unsprayed counterparts. From this study it was found that the indices of bacterial diversity, richness and evenness were found to be higher in phyllosphere receiving two sprays (on 15th and 30th) of Panchagavya as compared to single spray ad control samples.
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