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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1154-1167
DOI: https://doi.org/10.20546/ijcmas.2017.604.142


An Overview on Molecular Basis of Genetic Recombination
Mamta Nehra1*, Rajesh Kumar Sharma2 and Mukesh Choudhary3
1G B Pant University of Agriculture and Technology, Pantnagar- 263145, India
2ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
3ICAR-Indian Institute of Maize Research, Ludhiana- 141 004, India
*Corresponding author
Abstract:

Recombination is a process by which pieces of DNA are broken and recombined to produce new combinations of alleles. In 1964, Robin Holliday proposed a model for understanding molecular basis of recombination that accounted for heteroduplex formation and gene conversion during recombination. A new modified major model for recombination was given by Jack Szostak and colleagues in 1983, it is called the double-strand-break model. The initial steps in finding enzymes that carry out recombination were genetic screens for mutants of E. coli that are defective in recombination. One of the major pathways for generating 3’ single-stranded termini uses the RecBCD enzyme. The pairing of the two recombining DNA molecules (synapsis) and invasion of a single strand from the initiating duplex into the other duplex are both catalyzed by the multi-functional protein RecA. RuvA tetramers recognize the Holliday junction, and RuvB uses the energy of ATP hydrolysis to unwind the parental duplexes and form heteroduplexes between them. Ruv C is the endonuclease that cleaves the Holliday junctions. In eukaryotes, spo11 protein introduces DSBs in chromosomal DNA at many locations to initiate meiotic recombination. MRX protein processes the cleaved DNA ends for assembly of the RecA-like strand exchange proteins.  Dmc1/ Rad51 is a RecA-like protein that specifically functions in meiotic recombination.


Keywords: Recombination, Holliday model, DSB model, RecBCD enzyme.
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How to cite this article:

Mamta Nehra, Rajesh Kumar Sharma and Mukesh Choudhary. 2017. An Overview on Molecular Basis of Genetic RecombinationInt.J.Curr.Microbiol.App.Sci. 6(4): 1154-1167. doi: https://doi.org/10.20546/ijcmas.2017.604.142