National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Root-knot nematode (Meloidogyne spp.) is the most important nematode with a wide range of host. Pomegranate cultivation has been challenged by a complex of nematode disease complex that involves both root knot nematode and a fungal organism. This study was aimed at identifying the root knot nematode, Meloidogyne species and the fungal organism that cause wilt disease in pomegranate. Based on the morphological means using posterior cuticular pattern and molecular characterization using internal transcribed spacer (TW81-AB28) region tools, the root knot nematode was identified as M. incognita. The fungal causal organism of pomegranate wilt was identified as Fusarium species by conidial and hyphal structures and confirmed by molecular means by amplifying the internal transcribed spacer (ITS) region of the conserved ribosomal DNA using primers ITS1 and ITS4.
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