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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1087-1091
DOI: https://doi.org/10.20546/ijcmas.2017.604.135


Molecular Detection of Brucella Organism from Milk and Milk Products
Praveshika Dubey2, Kirit B. Patel1*, B.K. Patel1, H.C. Chauhan1, B.S. Chandel1,
S.S. Patel1, M.D. Shrimali1, J.K. Kala1, M.G. Patel1, A.C. Patel1,
Manish Rajgor1, M.A. Patel1 and A.N. Modi1
1Department of Animal Biotechnology and Veterinary Microbiology, College of Veterinary Science and  Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University,  Sardarkrushinagar- 385 506, Gujarat, India
2Department of Biotechnology, Hemchandracharya North Gujarat University, Patan, India
*Corresponding author
Abstract:

Brucellosis is the most common zoonosis in the world, Brucellosis is an infectious disease caused by bacteria of the genus Brucella. The disease has a considerable impact on human and animal health, as well as socioeconomic impacts, especially in which rural income relies largely on livestock breeding and dairy products. The disease can also be transmitted by direct or indirect contact with infected animals. Humans are commonly infected through ingestion of raw milk, milk product, meat or through direct contact with infected animals. In presence study was undertaken for the molecular detection of brucella organism from milk and milk products from different places of Gujarat. Out of 85 milk samples, 23 (27.05%) samples were found positive for Brucella antibodies by Milk Ring Test (MRT). A total of 168 samples (145 milk product and 23 MRT positive milk samples) processed for detection of Brucella organism using genus specific (B4/B5 primer) PCR. Out of 168 samples, 14 samples amplicon at 223bp by Brucella genus specific B4/B5primers indicates that all fourteen samples positive for Brucella organism.


Keywords: Milk Ring Test (MRT), Brucella, DNA Extraction, PCR, Milk product
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How to cite this article:

Praveshika Dubey, Kirit B. Patel, B.K. Patel, H.C. Chauhan, B.S. Chandel, S.S. Patel, M.D. Shrimali, J.K. Kala, M.G. Patel, A.C. Patel, Manish Rajgor, M.A. Patel and Modi, A.N. Molecular Detection of Brucella Organism from Milk and Milk Products.Int.J.Curr.Microbiol.App.Sci. 6(4): 1087-1091. doi: https://doi.org/10.20546/ijcmas.2017.604.135