'' '' '' '' Capsular Typing of Staphylococcus aureus Isolated from Raw Milk Samples using Duplex Polymerase Chain Reaction
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles                      Volume : 9, Issue:11, November, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(11): 2044-2050
DOI: https://doi.org/10.20546/ijcmas.2020.911.243


Capsular Typing of Staphylococcus aureus Isolated from Raw Milk Samples using Duplex Polymerase Chain Reaction
A. Khan1*, R. Sharma1, R. Grover1 and M. Rizwan2
1Department of Biotechnology, Mahatma Jyoti Rao Phoole University, Jaipur, Rajasthan, India
2Multi-Disciplinary Research Unit, S.P. Medical College, Bikaner, Rajasthan, India
*Corresponding author
Abstract:

The present investigation was designed to determine the frequency of capsular polysaccharide genes (cap5 & cap8) in Staphylococcus aureus strains isolated from raw milk samples of various regions of Jaipur, the capital city of Rajasthan state of India. In this study presence of S. aureus and cap5and cap8 gene was evaluated by Polymerase Chain Reaction. A sum of 59 strains of S. aureus (confirmed by 23S rRNA amplification) were selected and screened for the presence of capsular genes. PCR amplification of the gene segment encoding cap5 and cap8 gene yielded product size of 361 and 173 bp, respectively. In the present study total 54.24% strains were found to carry cap5 gene whereascap8 gene was found with 27.12% prevalence. Presence of both genes was found is 10.17% strains. Maximum prevalence (66.76%) of cap5 gene was present in the strains of Amer and Bagru regions while lowest prevalence (37.50%) was found in the strains of Durgapuraregion of Jaipur city. Further, prevalence of cap8 gene was maximum (42.86%) found in strains of Sodala area which is surprisingly as similar as cap5 gene and was lowest in strains of Amer area (11.11%). 25% of the strains of Durgapura area were not typable. This study revealed that presence of cap5 gene was higher than cap8 gene and also provides an idea that infection of S. aureus may pose a potential risk to human health and these result may support the future strategies and actions related to milk safety programs.


Keywords: Staphylococcus aureus, Milk, cap5, cap8
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How to cite this article:

Khan, A., R. Sharma, R. Grover and Rizwan, M. 2020. Capsular Typing of Staphylococcus aureus Isolated from Raw Milk Samples using Duplex Polymerase Chain Reaction.Int.J.Curr.Microbiol.App.Sci. 9(11): 2044-2050. doi: https://doi.org/10.20546/ijcmas.2020.911.243
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.