National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present investigation was designed to determine the frequency of capsular polysaccharide genes (cap5 & cap8) in Staphylococcus aureus strains isolated from raw milk samples of various regions of Jaipur, the capital city of Rajasthan state of India. In this study presence of S. aureus and cap5and cap8 gene was evaluated by Polymerase Chain Reaction. A sum of 59 strains of S. aureus (confirmed by 23S rRNA amplification) were selected and screened for the presence of capsular genes. PCR amplification of the gene segment encoding cap5 and cap8 gene yielded product size of 361 and 173 bp, respectively. In the present study total 54.24% strains were found to carry cap5 gene whereascap8 gene was found with 27.12% prevalence. Presence of both genes was found is 10.17% strains. Maximum prevalence (66.76%) of cap5 gene was present in the strains of Amer and Bagru regions while lowest prevalence (37.50%) was found in the strains of Durgapuraregion of Jaipur city. Further, prevalence of cap8 gene was maximum (42.86%) found in strains of Sodala area which is surprisingly as similar as cap5 gene and was lowest in strains of Amer area (11.11%). 25% of the strains of Durgapura area were not typable. This study revealed that presence of cap5 gene was higher than cap8 gene and also provides an idea that infection of S. aureus may pose a potential risk to human health and these result may support the future strategies and actions related to milk safety programs.
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