National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was aimed to develop a most effective extender for long term storage of Beetal buck semen at 4℃. Tris-citric acid-fructose was supplemented with egg yolk (TEY), egg yolk plasma (TEYP), @ 5, 10, 15 and 20; 5, 10, 15 and 20 percent, respectively. Extended semen was stored at 4℃ and analyzed for sperm attributes. Values for motility, viability and plasma membrane integrity and acrosomal integrity remained significantly (p<0.05) higher in 15% TEY, 15% TEYP from 0 hr to 72 hrs of storage. It indicated superiority of 15% TEY, 15% TEYP, over other concentrations for storage of beetal buck semen at 4℃. A significant (p < 0.05) decline in motility, viability and membrane integrity was observed during 0-72 hrs but sperm attributes were still >50% at 72 hrs of preservation. Though, both types of extenders at 15% level of concentration were able to sustain sperm attributes to>60% level at 72 hrs of preservation. Percent decline in sperm attributes was recorded comparatively less at 15 % concentration in both type of extenders. Values for acrosome integrity also differed significantly (p<0.05) among different concentrations of both types of extenders. In conclusion, both 15% TEY and 15% TEYP were better than other concentrations.
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