National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Chickpea (Cicer arietinum L., 2n=16) is a legume of family Fabaceae, subfamily Faboideae. Chickpea is the second largest grown food legume of the world (Gaur et al., 2008). Genetic divergence among 28 genotypes of Cicer arietinum L. was assessed through RAPD molecular markers. RAPD analysis showed 93.36 per cent of polymorphism. A total of 159 amplified bands were obtained that ranged between 150 bp to 3200 bp, using 22 RAPD primers only 15 showed amplification. The 153 out of 159 bands were polymorphic. The average PIC (polymorphic information content) of RAPD was found 0.259. Jaccard’s similarity coefficient for RAPD lay between 0.31 and 0.79 (31% to 79%). Dendogram based on genetic distance calculated by UPGMA method segregated the 28 chickpea genotypes into three RAPD major clusters, respectively. Two and three dimension principal component analysis (PCA) showed similar clustering of 28 genotypes as evident from cluster tree analysis with dice similarity coefficients ranged from 0.58 to 0.85 (58% to 85%) for RAPD analysis. Based upon RAPD analysis of 28 genotypes with 15 primers genotype PBC-509, PBC-511, Pratap channa-1, Dohad yellow and Pratap Rajchana were identified as genetically diverse. Therefore, the results of the present study can prove helpful for implementation of chickpea improvement programmes.
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