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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 129-136
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.018


Prevalence, Bacteriology, Pathogenesis and Isolation of E. coli in Sick Layer Chickens in Ajmer Region of Rajasthan, India
Tripti Dadheech1*, Reena Vyas1, Vijaylatha Rastogi2
1Department of Zoology, Govt. College Ajmer, India
2Microbiology Department, Jawahar Lal Nehru Medical College, Ajmer, India
*Corresponding author
Abstract:

A study was conducted to determine the distribution of E. coli in visceral organs of clinically sick chickens (Gallus gallus). A total of 24 tissue samples consisting of 6 liver, 12 small intestine, and 6 large intestine samples were aseptically collected from 12 fowls of 4 selected poultry farms of Ajmer suspected for colibacillosis and subjected to bacteriology and biochemical examination. 24 E. coli isolates were isolated following standard procedures. Processed tissue from each sample was cultured primarily on Brain Heart Infusion (BHI) broth and then secondarily on 7% defibrinated sheep blood (Blood Agar) and MacConkey agar. Presumptive colonies of bacterial agents on media plates were subjected to conventional biochemical characterization. The result of biochemical test identified the E. coli isolates from the samples of clinically sick chickens, almost 100%. The overall prevalence of E. coli was 100% and high rate of infection.


Keywords: Collibacillosis, Bacteriology, Biochemical Characterization, Prevalence, Infection.
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How to cite this article:

Tripti Dadheech, Reena Vyas, Vijaylatha Rastogi. 2016. Prevalence, Bacteriology, Pathogenesis and Isolation of E. coli in Sick Layer Chickens in Ajmer Region of Rajasthan, India.Int.J.Curr.Microbiol.App.Sci. 5(3): 129-136. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.018