National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Orexin A and B are neuropeptides synthesized mainly in the lateral hypothalamus and are associated with a variety of physiological functions such as energy homeostasis, sleep and wakefulness, feeding behavior, as well as the reproductive system. The biological actions of the hormones are mediated via two distinct G protein-coupled receptors, termed orexin receptor 1 (OX1R) and orexin receptor 2 (OX2R). The aim of the current study was to investigate the gene expression of orexin and its receptors OX1R and OX2R in different stages of corpus luteum (CL) during estrous cycle in (Bubalus bubalis). The result showed that transcript of pre-pro-orexin (PPO), OX1R and OX2R was present in buffalo CL throughout the estrous cycle however the level of transcript varied through different stages of CL. The abundance of transcript of orexin and its receptor was high higher P<0.05) in early (CL1, days 1-4) and regressing stage (CL4, days >17) of CL whereas the expression was significantly lower (P<0.05) in mid (CL2, days 5-10) and late luteal (CL3, days 11-16) stage during estrous cycle. Presence of both ligand (orexin) and receptors (OX1R) and (OX2R) in CL of buffalo ovary suggest the autocrine and paracrine role of orexin in corpus luteum functions and indicate the role of orexin in regulation of reproductive functions in water buffalo.
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