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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 938-947
DOI: https://doi.org/10.20546/ijcmas.2017.604.118


Biosynthesis and Characterization of Silver Nanoparticles
Payal N. Agrawal and Nikhilesh S. Kulkarni
2Department of Microbiology, Microbiology Research Laboratory, R.A. College, Washim-444505 (M.S.), India
*Corresponding author
Abstract:

The development of reliable, eco-friendly process for the synthesis of nanoparticles is an important aspect of nanotechnology. In the present study, the extracellular synthesis of Silver nanoparticles was done by reducing aqueous Ag+ with the culture supernatant of four different bacterial strains viz. Bacillus flexus, Bacillus pseudomycoides, Cronobacte runiversalis, and Kocuria rosea. The formation of silver nanoparticles was confirmed by the change in colour from colorless to brown. The synthesized nanoparticles were characterized adopting suggested instrumentations. In case of UV-Visible spectroscopy, four strong peaks were observed at 430, 410, 420 and 420 nm respectively which confirmed the synthesis of AgNPs.  FTIR analysis confirms the presence of elemental silver and reveals the dual function of biological molecule responsible for the reduction and stabilization of AgNPs in the aqueous medium. The XRD showed that silver nanoparticles produced are crystalline in nature with size ranges from 30 to 70 nm. The SEM, shows that produced silver nanoparticles are spherical, Pseudo spherical in shape. However, it also showed an indeterminate morphology. The silver nanoparticle also showed traces of agglomeration. Silver nanoparticles synthesized through this biosynthesis method have been known to posses’ great interest in recent times due to their reported advantageous properties as well as application in a diversity of fields.


Keywords: Biosynthesis, Silver Nanoparticles, Bacillus flexus, Bacillus pseudomycoides, Cronobacter universalis  and Kocuria rosea.
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How to cite this article:

Payal N. Agrawal, Nikhilesh S. Kulkarni. 2017. Biosynthesis and Characterization of Silver NanoparticlesInt.J.Curr.Microbiol.App.Sci. 6(4): 938-947. doi: https://doi.org/10.20546/ijcmas.2017.604.118