National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Haemophilus influenzae is strictly a human pathogen, responsible for many diseases like community acquired pneumonia, meningitis, sinusitis, epiglottitis and otitis media. Haemophilus type b (Hib) was the most common strain responsible for all the infections. Non type b strains were at an emerging risk. Ampicillin was commonly used but resistance due to TEM-1gene is being reported. It is aimed to find out whether Hib continues to be isolated from childhood cases of pneumonia and its susceptibility to the drugs of choice and possible mechanisms of resistance. All purulent respiratory samples were processed for isolation of Haemophilus influenzae. They were identified based on its X&V factor requirement and a negative Porphyrin synthesis test. Biotyping and Serotyping was done using standard methods. Ampicillin resistance was characterized by phenotypic and genotypic methods. From the results, out of 1540 purulent respiratory samples, Haemophilus influenzae was isolated from 103 cases. Among the 103 isolates, 69 were Nontype b and 34 were type b Haemophilus influenzae. All the Hib isolates were from those above 20 years of age. Among 15 Ampicillin resistant isolates, 13 were positive for TEM-1 gene. Finally, Nontype b isolates were predominant and Hib was isolated only from adult age group.
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