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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 929-937
DOI: https://doi.org/10.20546/ijcmas.2017.604.117


Haemophilus influenzae in Community Acquired Infections
Sai Keerthana* and Marina Thomas Appalaraju
Department of Microbiology, PSG IMS&R, Coimbatore, India
*Corresponding author
Abstract:

Haemophilus influenzae is strictly a human pathogen, responsible for many diseases like community acquired pneumonia, meningitis, sinusitis, epiglottitis and otitis media. Haemophilus type b (Hib) was the most common strain responsible for all the infections. Non type b strains were at an emerging risk. Ampicillin was commonly used but resistance due to TEM-1gene is being reported. It is aimed to find out whether Hib continues to be isolated from childhood cases of pneumonia and its susceptibility to the drugs of choice and possible mechanisms of resistance. All purulent respiratory samples were processed for isolation of Haemophilus influenzae. They were identified based on its X&V factor requirement and a negative Porphyrin synthesis test. Biotyping and Serotyping was done using standard methods. Ampicillin resistance was characterized by phenotypic and genotypic methods. From the results, out of 1540 purulent respiratory samples, Haemophilus influenzae was isolated from 103 cases. Among the 103 isolates, 69 were Nontype b and 34 were type b Haemophilus influenzae. All the Hib isolates were from those above 20 years of age. Among 15 Ampicillin resistant isolates, 13 were positive for TEM-1 gene. Finally, Nontype b isolates were predominant and Hib was isolated only from adult age group.


Keywords: Hib, Non type b, Porphyrin synthesis test, X&V factors, TEM-1.
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How to cite this article:

Sai Keerthana, Marina Thomas Appalaraju. 2017. Haemophilus influenzae in Community Acquired InfectionsInt.J.Curr.Microbiol.App.Sci. 6(4): 929-937. doi: https://doi.org/10.20546/ijcmas.2017.604.117