National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions is presented in this paper. Shoot–tip cultures of Musa Cultivator (both banana and plantain) are induced by culturing small excised shoot apices on modified MS semisolide medium supplements with various concentration and combination of auxins and cytocynins. The explants were selected from the field of Ishved Biotech PVT.LTD. Sindhkedraja Dist. Buldana for the experiments. Present investigation entitled “Effect of pH on shoot proliferation of banana (Musa sp.) with constant BAP (4mg/l).”There were five treatments of pH used in that experiments i.e. pH 5.2, 5.5, 5.8, 6.1, 6.4 and media prepared with different treatments of pH and with constant BAP. 1L media per treatment. BAP is constant 4mg/L. This media in bottles, and kept for incubation of one day. The healthy, disease free suckers as explants were selected for the experimentation. The sterilization of explants was done with different sterilization agents in with ethanol, ascorbic acid, mercuric chloride streptocyclin, tween -20 bavistin then inoculation of explants was done under laminar air flow. Inoculated bottles are transferred to growth room for growth are under controlled condition, temp is maintained 25+/_ 2 0C with 16hr photoperiod.
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