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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:11, November, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(11): 623-628
DOI: https://doi.org/10.20546/ijcmas.2020.911.076


Confirmation of Hybridity using DNA-based Markers is Essential in Chickpea (Cicer arietinum L.)
Sanchit Thakur1, Jai Dev Sharma2* and Kamal Dev Sharma3
1Department of Plant Breeding and Genetics,
2Department of Tea Husbandry & Technology,
3Department of Agricultural Biotechnology, CSKHPKV, Palampur, India
*Corresponding author
Abstract:

Confirmation of hybridity is essential to exclude selfed plants in plant breeding programmes. The morphological markers for confirmation of hybridity in chickpea are unavailable in closely related germplasm and these results in ambiguity in identification of true hybrids. In present study, cold tolerant parent ICC-16349 (donor) and cold susceptible parent GPF-2 (recipient) were crossed to generate 80 putative hybrids. The parents were screened using 51 simple sequence repeat (SSR) markers, of which only one i.e TA 180 exhibited polymorphism. Screening of 80 putative hybrids using TA 180 revealed that only 34 (42.5%) of the putative hybrids were true hybrids. The study indicated that in chickpea breeding programmes, hybridity must be confirmed using DNA based markers to avoid inclusion of selfed plants as hybrids.


Keywords: Chickpea, Hybridization, SSR, True hybrids

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How to cite this article:

Sanchit Thakur, Jai Dev Sharma and Kamal Dev Sharma. 2020. Confirmation of Hybridity using DNA-based Markers is Essential in Chickpea (Cicer arietinum L.).Int.J.Curr.Microbiol.App.Sci. 9(11): 623-628. doi: https://doi.org/10.20546/ijcmas.2020.911.076
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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