National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present investigation was carried out to study the prevalence of bacterial pathogens responsible for subclinical mastitis in buffaloes in and around Pantnagar in the state of Uttarakhand, India and the antibiogram pattern of the bacterial isolates to selected antibiotics. A total of 109 buffaloes were screened for SCM based on physical examination of udder, California mastitis test (CMT), Somatic cell count (SCC) and Differential cell count (DCC) on quarter’s milk samples. The quarter’s milk samples showing CMT reaction ≥ 2, SCC ≥ 0.5 million/ml and neutrophils percentage ≥ 40% of total counts were considered for identification of major pathogens involved. Milk samples from affected quarters were subjected to cultural and biochemical examination for isolation and identification of bacterial pathogens. Antibiogram profile of each bacterial isolate to standard antimicrobial discs was determined by agar diffusion method. Staphylococcus aureus were the most common isolates of subclinical mastitis in dairy buffaloes, followed by streptococci other than S. agalactiae, Coagulase negative staphyllococus and S. agalactiae isolates respectively. The overall antibiogram of the major bacterial isolates revealed the highest sensitivity to enrofloxacin (86.5%), followed by cefotaxime (82.0%) and amoxycillin/clavulanic acid (80.9%). The current work enables selection of proper antibacterials for treatment of mastitis in the study area.
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