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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 3461-3474
DOI: https://doi.org/10.20546/ijcmas.2020.910.400


Optimal Conditions for Clostridium difficile Toxins Production
Medhat M. Taha*
Veterinary Serum and Vaccine Research Institute, Agriculture Research Center (ARC), Abbasia, Cairo, Egypt
*Corresponding author
Abstract:

Clostridium difficile causes acute enterocolitis among different animal species. The disease is mediated primarily through two exotoxins known as TcdA and TcdB, their synthesis depends mainly on growth of the organism which is controlled by many factors. Variations in the production of these toxins are affected by the medium and its nutrients, the using of carbon source in toxin production medium will affect the toxin level. In this study , we use different sources of carbon in the toxin production media , It was found that glucose and fructose gave low toxin production levels while using 1%starch in toxin production media for 5 days incubation and then separation of the produced toxins using 0.22um Millipore filter , high level of Clostridium difficile toxins obtained that could be used in the future for preparation of formalized toxoid against enterocollitis from locally isolated strain of  Clostridium difficile .


Keywords: Clostridium difficile,Toxins
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How to cite this article:

Medhat M. Taha. 2020. Optimal Conditions for Clostridium difficile Toxins Production.Int.J.Curr.Microbiol.App.Sci. 9(10): 3461-3474. doi: https://doi.org/10.20546/ijcmas.2020.910.400