National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Clostridium difficile causes acute enterocolitis among different animal species. The disease is mediated primarily through two exotoxins known as TcdA and TcdB, their synthesis depends mainly on growth of the organism which is controlled by many factors. Variations in the production of these toxins are affected by the medium and its nutrients, the using of carbon source in toxin production medium will affect the toxin level. In this study , we use different sources of carbon in the toxin production media , It was found that glucose and fructose gave low toxin production levels while using 1%starch in toxin production media for 5 days incubation and then separation of the produced toxins using 0.22um Millipore filter , high level of Clostridium difficile toxins obtained that could be used in the future for preparation of formalized toxoid against enterocollitis from locally isolated strain of Clostridium difficile .
Citations |
 |
 |
 |
 |
