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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 2952-2961
DOI: https://doi.org/10.20546/ijcmas.2020.910.356


Conjunctival Immunocytochemistry with Interferon Gamma and Caspase 3 for Clinical Staging of Keratoconjunctivitis Sicca (KCS) in Dogs
Christina John1, Aswathy Gopinathan2*, Kiranjeet Singh3, Chelladurai Sowbharenya1, Naveen Kumar2, Monalisa Sahoo3 and Ravi Kant Agrawal1
1LPT Division, Council of Agricultural Research- Indian Veterinary Research Instistute, Izzatnagar, Barielly, Uttar Pradesh, India
2Division of Surgery, 3Division of Pathology LPT Division, Council of Agricultural Research- Indian Veterinary Research Instistute, Izzatnagar, Barielly, Uttar Pradesh, India
*Corresponding author
Abstract:

The study was conducted on 67 client-owned dogs affected with Keratoconjunctivitis Sicca (KCS) to evaluate the expression of Caspase-3 and Interferon gamma in conjunctival cells.  After complete ophthalmic examinations cases were staged into Early (E), Late (L) and ulcer group (R). Conjunctival scrapings for immunocytochemical expression of Caspase-3 and INF gamma and tissue samples for histopathological confirmation of KCS were collected from early KCS, late KCS and apparently healthy animals. The prevalence of KCS cases was found to be 3.33% (67/1997) in dogs. Corneal ulcers (R) due to KCS constituted 38 % (27), early KCS 36% (24) and late KCS 24% (16) among cases. Cases with early and late stages of KCS were having significantly (0.05%) high expression for Caspase 3 and INF gamma as compared to apparently healthy animals.  In Group E, 89.33% and 85.04% of cells were found positive for Caspase -3 and interferon gamma, respectively. In group L 25.57% and 85.05% of cells were found positive for Caspase -3 and interferon gamma, respectively. Immunocytochemical findings of present study corroborated T cell proliferation and apoptosis as major pathological events at different stages of KCS.


Keywords: Caspase -3, Dogs, Immuno cytochemistry, Interferon- gamma, Keratoconjunctivitis sicca
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How to cite this article:

Christina John, Aswathy Gopinathan, Kiranjeet Singh, Chelladurai Sowbharenya, Naveen Kumar, Monalisa Sahoo and Ravi Kant Agrawal. 2020. Conjunctival Immunocytochemistry with Interferon Gamma and Caspase 3 for Clinical Staging of Keratoconjunctivitis Sicca (KCS) in Dogs.Int.J.Curr.Microbiol.App.Sci. 9(10): 2952-2961. doi: https://doi.org/10.20546/ijcmas.2020.910.356