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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 2272-2280
DOI: https://doi.org/10.20546/ijcmas.2020.910.275


Development of Multiplex Microsatellite Marker Setsin Soybean [Glycine max (L.) Merr.]
Shipra Deo1, Anto James1, Sanjeev Kumar Singh1C.B. Singh2 and Mukesh Kumar Rana1*
1ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110 012
2Department of Botany, TilkaManjhi Bhagalpur University, Bhagalpur, Bihar812007, India
*Corresponding author
Abstract:

Multiplex PCR (mPCR) analyzes several markers simultaneously in a single reaction. The development of an SSR multiplex system requires estimation of range of allele sizes of different markers for their grouping into multiplex sets. In the present investigation duplex, triplex, quadruplex, and pentaplex combinations of SSR markers were optimized for mPCR using four different soybean genotypes namely, JJS 335, Durga, MACS 124 and JS 90-41.Twenty SSR markers distributed across all linkage groups of soybean were used to develop 22 multiplex sets of markers. These 22 multiplex sets comprised of twelve duplex, five triplex, four quadruplex and one pentaplex assays. Each band of mPCR was considered a single genetic marker and using all mPCR marker sets bands in the size range of 100-300bp were observed. The methodology used was quite systematic, rapid, reliable, and appropriate for qualitative detection and can be extended for multiplex development in other plant species having SSR marker technology for efficient plant genetic resources management including genetic diversity analysis, cultivar identification and evolutionary studies in plants.


Keywords: Multiplex PCR, PCR optimization, Glycine max L., SSR markers
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How to cite this article:

Shipra Deo, Anto James, Sanjeev Kumar Singh, C.B. Singh and Mukesh Kumar Rana. 2020. Development of Multiplex Microsatellite Marker Sets in Soybean [Glycine max (L.) Merr.].Int.J.Curr.Microbiol.App.Sci. 9(10): 2272-2280. doi: https://doi.org/10.20546/ijcmas.2020.910.275