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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 2164-2173
DOI: https://doi.org/10.20546/ijcmas.2020.910.263


Metagenomic Analysis of Bacterial Diversity on Tomato (Solanum lycopersicum L.) Leaves
Sumbula1*, P. Sainamole Kurian1, D. Girija1 and K. Anita Cherian1
1Department of Plant Pathology, 2Department of Agricultural Microbiology, College of Horticulture, Kerala Agricultural University, Thrissur 680656, Kerala, India
*Corresponding author
Abstract:

Plant leaves are colonized by complex microbial communities, which are adapted to the peculiar habitat on leaf lamina. Although the role and ecology of microorganisms in and on the leaves are only partially understood, leaf microbiota could have a beneficial role in plant growth and health. An amplicon metagenomic approach based on 16SrRNA gene on the MiSeq Illumina platform was used to identify the composition of bacterial communities associated with tomato leaves. A total of 44380 high quality 16s DNA sequences were clustered into 365 operational taxonomic units (OTUs). The results of metagenomomic analysis revealed that, tomato leaf bacterial community mainly comprised five dominant and four rare phyla, among which, Firmicutes (109 OTUs) was the most represented followed by Proteobacteria (95 OTUs), Cyanobacteria (46 OTUs), Bacteriodetes (43 OTUs) and Actinobacteria (16 OTUs). Rare phyla include, Verrucomicrobia (3 OTUs), Acidobacteria (2 OTUs) and Gemmatimonadetes (1 OTUs). Clostridia and Gammaproteobacteria was the most abundant classes of Firmicutes and Proteobacteria respectively. The dominant genera were Geitlerinema (33 OTUs), Pantoea(31 OTUs), Clostridium (26 OTUs) and Acinetobacter (24 OTUs). Besides the presence of common commensal leaf bacteria viz. Pantoea Sphingomonas, and Acinetobacter, the occurrence of Clostridium, a food, animal and human pathogenic genus and Geitlerinema, a fresh and marine water cyanobacteria on tomato leaves is an important observation of the present study. As a whole, this study describes the composition of the bacterial communities of tomato leaves, identifying a variety of genera that could exert multiple effects on growth and health of tomato plants.


Keywords: Illumina platform, Metagenomics, Operational taxonomic units (OTUs), 16SrRNA, Tomato
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How to cite this article:

Sumbula, V., P. Sainamole Kurian, D. Girija and Anita Cherian, K. 2020. Metagenomic Analysis of Bacterial Diversity on Tomato (Solanum lycopersicum L.) Leaves.Int.J.Curr.Microbiol.App.Sci. 9(10): 2164-2173. doi: https://doi.org/10.20546/ijcmas.2020.910.263