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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 2601-2608
DOI: https://doi.org/10.20546/ijcmas.2020.910.313


Determination of Lethal Dose (LD50) and Effects of Gamma Rays and Ethyl Methane Sulphonate (EMS) Induced Mutagenesis in Linseed (Linum usitatissimum L.)
Arjun Kumar*, Satish Paul and Garima Thakur
Department of Crop Improvement, College of Agriculture, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur (HP), India
*Corresponding author
Abstract:

The present investigation was undertaken to study the effect of non particulate gamma radiation and EMS mutagens on linseed varieties viz., Baner, Him Alsi-2 and Surbhi. Many significant effects of EMS concentration and gamma rays dosages on seed germination were observed. The percent germination was reduced from 84, 86 and 80 to 65, 58 and 59 percent with 60 kR (kilo Roentgen) for cultivars Baner, Him Alsi-2 and Surbhi respectively. LD50 concentration of gamma rays varies in different varieties viz., 96.63 kR (Baner), 72.94 kR (Him Alsi-2- and 53.22 kR (Surbhi). The percent germination was reduced from 87, 94 and 79 to 49, 50 and 10 percent with 0.8 percent EMS for cultivars Baner, Him Alsi-2 and Surbhi respectively. Also, lethality (LD50) of EMS between varieties varies i.e., Baner (0.86%), Him Alsi- 2 (0.72%) and Surbhi (0.35%) showing that lethality depends upon the characteristics of seed. Variable effects were observed in field condition for seed germination and survival in all the varieties indicating that these mutagens can be used in improvement of particular characteristics of crop by following proper selection methods.


Keywords: Mutation, Mutagens, Gamma rays, EMS
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How to cite this article:

Arjun Kumar, Satish Paul and Garima Thakur. 2020. Determination of Lethal Dose (LD50) and Effects of Gamma Rays and Ethyl Methane Sulphonate (EMS) Induced Mutagenesis in Linseed (Linum usitatissimum L.).Int.J.Curr.Microbiol.App.Sci. 9(10): 2601-2608. doi: https://doi.org/10.20546/ijcmas.2020.910.313