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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 2534-2451
DOI: https://doi.org/10.20546/ijcmas.2020.910.305


Identification of Green Tissue Specific Genes in Cotton Employing Transcriptome Sequencing
D. Anne Kitty Deborah1*, Ganesan Balasubramani2 and Vijay N. Waghmare3
1Women Scientist Scheme-A of Department of Science and Technology, Biotechnology unit, ICAR-Central Institute for cotton Research, Post Bag No-2, Shankar nagar post office, Nagpur-440010, Maharashtra
2Biotechnology unit, ICAR-Central Institute for cotton Research, Post Bag No-2, Shankar nagar post office, Nagpur-440010, Maharashtra
3ICAR
Central Institute for cotton Research, Post Bag No-2, Shankar nagar post office, Nagpur-440010, Maharashtra, India
*Corresponding author
Abstract:

The present study aims at identification of green tissue specific genes in Gossypium hirsutum variety, Suraj for promoter isolation and characterization in the future. For this, transcriptome sequencing of three different tissues viz., seed, leaf and boll rind was done. Differential gene expression analysis between seed (non green) and leaf and bollrind (green) tissues was done to identify genes which are highly expressed in green tissues i.e., leaf and boll rind and very low or no expression in non green tissues i.e., seed. Fifty genes were selected for validation through Real Time PCR studies. For Real Time PCR studies, apart from leaf , boll rind and seed at 25 DPA, boll rind and seed @ 10 DPA, pollen and petals were also studied. Five genes were found to show high expression in green tissues and very low or no expression in non green tissues. As expected, most of them were found to be chloroplastic genes. These are the potential genes for isolation and characterization of green tissue specific promoters.


Keywords: Transcriptome sequencing, Differential gene expression, Green tissue
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How to cite this article:

Anne Kitty Deborah, D., Ganesan Balasubramani and Vijay N. Waghmare. 2020. Identification of Green Tissue Specific Genes in Cotton Employing Transcriptome Sequencing.Int.J.Curr.Microbiol.App.Sci. 9(10): 2534-2451. doi: https://doi.org/10.20546/ijcmas.2020.910.305