National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study aims at identification of green tissue specific genes in Gossypium hirsutum variety, Suraj for promoter isolation and characterization in the future. For this, transcriptome sequencing of three different tissues viz., seed, leaf and boll rind was done. Differential gene expression analysis between seed (non green) and leaf and bollrind (green) tissues was done to identify genes which are highly expressed in green tissues i.e., leaf and boll rind and very low or no expression in non green tissues i.e., seed. Fifty genes were selected for validation through Real Time PCR studies. For Real Time PCR studies, apart from leaf , boll rind and seed at 25 DPA, boll rind and seed @ 10 DPA, pollen and petals were also studied. Five genes were found to show high expression in green tissues and very low or no expression in non green tissues. As expected, most of them were found to be chloroplastic genes. These are the potential genes for isolation and characterization of green tissue specific promoters.
Citations |
 |
 |
 |
 |
