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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 2133-2144
DOI: https://doi.org/10.20546/ijcmas.2020.910.260


Effect of Peripheral Blood Mononuclear Cells on Induced Ischemia/Reperfusion in Skeletal Muscle of Adult Male Albino Rat: An Immunohistochemical Study
Hend I. Abd El Halim*, Reda H. Elbakary, Ebtsam F. Okasha,
Amany M. Mousa and Ehsan F. Salah
Histology Department, Tanta University, Tanta, Egypt
*Corresponding author
Abstract:

Lower limbs ischemia is a life-threatening condition resulting from hypovolemia or vascular occlusion. Peripheral blood mononuclear cells (PBMCs) may represent a novel treatment for ischemia reperfusion injury (IRI).This work was performed to study the effect of PBMCs on induced ischemia /reperfusion in skeletal muscle of adult male albino rat.  Fifty adult male albino rats were used; twenty were considered as donors and the others as recipients. The recipients were divided into three groups. Group I served as a control. Group II used for induction of IRI; half of rats was sacrificed immediately (subgroup II-A) and the other half after 4 weeks (subgroup II-B).Group III: IRI induction was done then rats of subgroup III-A were injected with phosphate buffered saline while rats of subgroup III-B were treated by intramuscular injection of PBMCs. Rats of group III were sacrificed after 4 weeks. Muscle samples of all groups were processed for immunohistochemical study and morphological data were statistically analyzed. Rats treated with PBMCs showed well-evidenced repair of the skeletal muscle IRI.


Keywords: Ischemia /reperfusion, Skeletal muscle, Peripheral blood mononuclear cells
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How to cite this article:

Hend I. Abd El Halim, Reda H. Elbakary, Ebtsam F. Okasha, Amany M. Mousa and Ehsan F. Salah. 2020. Effect of Peripheral Blood Mononuclear Cells on Induced Ischemia/Reperfusion in Skeletal Muscle of Adult Male Albino Rat: An Immunohistochemical Study.Int.J.Curr.Microbiol.App.Sci. 9(10): 2133-2144. doi: https://doi.org/10.20546/ijcmas.2020.910.260