The purpose of this research was to evaluate antagonistic activity of Bacillus subtilis isolated from Phylloplane and rhizospheric soil of different field crops. Bacteria are able to synthesis a wide range of secondary metabolites with fungicidal capabilities. The antagonistic potential of Bacillus subtilis was assessed in vitro against some foliar and soil borne plant pathogenic fungi. The result indicated that tested phylloplane Bacillus subtilis isolates exhibited antagonistic potential in the range of 76.13- 85.59% against foliar pathogenic fungi and also showed that rhizosphere Bacillus subtilis recorded between 57.16- 71.89% against soil borne pathogenic fungi. Thus results indicated that tested bacterial species can be used as promising biocontrol agent.

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