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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 1844-1851
DOI: https://doi.org/10.20546/ijcmas.2020.910.225


Formulated Sorghum Media as Alternative to Nutrient broth in Cultivation of Staphylococcus aureus (NCTC 6571) and Bacillus subtilis (NCTC 8241)
S. Izebe1, J. A. Onaolapo2, K. Ibrahim1, Y. K. E. Ibrahim2, P. Oladosu1,
Ya’aba1*, S. B. Mohammed1, P. O. Adigwe1 and P. F. Olurinola2
1Department of Microbiology and Biotechnology, National Institute for Pharmaceutical Research and Development, Idu, Abuja, Nigeria
2Department of Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria
*Corresponding author
Abstract:

Microbiological culture media are expensive been imported from developed countries, and there is need to formulate our local media from vast raw materials available to improve our foreign reserves. This study was aimed at formulation of culture media from sorghum grains to cultivate test bacteria commonly used in research purposes. The sorghum grains free from debris were soaked in distilled water for 24 hr. and ground into paste. The fibers were removed by filtration followed by centrifuged at 9000 rpm for 10 minutes to obtained gluten and starch deposits which were separated and dried at 25oC. The dried gluten and starch were digested with pepsin and amylase enzymes to obtained protein and sugar digests which was combined proportionally and pH.7.2 adjusted. A pure culture each of Staphylococcus aureus (NCTC 6571) and Bacillus subtilis (NCTC 8241) were inoculated into sterile formulated sorghum and nutrient media. A visualized turbidity was observed. The OD (0.66 and 0.65) of S. aureus and (0.57 and 0.47) for B. subtilis in sorghum and nutrient media respectively. The mean viable count of 6.0 x 1021 ± 0.004 and 5.7 x1021 ± 0.003 CFU/mL of Staphylococcus aureus in sorghum and nutrient media followed by 4.3 x1020 ± 0.003 and 4.7 x 1021 ± 0.005 CFU/mL of Bacillus subtilis in nutrient and sorghum media respectively. There is no significant difference in viable counts of bacteria (p<0.05) in these media. The growth curves exhibited by Staphylococcus aureus and Bacillus subtilis in sorghum and nutrient media showed similar pattern of no lag phase and lengthen log phase. The formulated sorghum media promotes and support the bacteria. The study recommends that sorghum media can be an alternative to nutrient broth as routine culture media.


Keywords: Formulated Sorghum media, Nutrient Broth, Viable count, Staphylococcus aureus, Bacillus subtilis, Culture media, Sorghum grains, Enzymes
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How to cite this article:

Izebe, K. S., J. A. Onaolapo, K. Ibrahim, Y. K. E. Ibrahim, P. Oladosu, Y. Ya’aba, S. B. Mohammed, P. O. Adigwe and Olurinola, P. F. 2020. Formulated Sorghum Media as Alternative to Nutrient broth in Cultivation of Staphylococcus aureus (NCTC 6571) and Bacillus subtilis (NCTC 8241).Int.J.Curr.Microbiol.App.Sci. 9(10): 1844-1851. doi: https://doi.org/10.20546/ijcmas.2020.910.225