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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 1756-1763
DOI: https://doi.org/10.20546/ijcmas.2020.910.213


Determination of Mutagenic Sensitivity in Hibiscus rosa-sinensis L. (Cultivar Red Single) to Physical and Chemical Mutagens
Naveena1*, S. Subramanian2, M. Jawaharlal3, K. Iyanar4 and C. N. Chandrasekhar5
1Department of Floriculture & Landscape Architecture, 2Horticultural College & Research Institute, 3Directorate of Extension Education, 4Department of Millets, Tamil Nadu Agricultural University, Coimbatore, India
5Agricultural College, Research Institute, Tamil Nadu Agricultural University, Coimbatore, India
*Corresponding author
Abstract:

Hibiscus, being an potential ornamental shrub needs improvement in terms of architecture. Mutation breeding technique has been considered as an efficient tool for vegetatively propagated crops. For every mutation breeding experiment, determination of mutagenic sensitivity is an important step because it varies with every species and varieties. Based on this, investigation on the induction of mutation in Hibiscus rosa-sinensis L. was carried out with the objective of creating genetic variability through physical mutagen viz., gamma rays and Ethyl Methane Sulphonate (EMS). In this study, hard wood cuttings of Hibiscus rosa-sinenis Cultivar Red Single were treated with nine different dose of gamma rays (1 to
5 k R) and ten different concentrations of EMS ranging from 0.1% to 1.0%. The results revealed that gradual reduction in survival of cuttings, shoot length, leaf length and leaf width with increase in dosage of gamma rays and EMS. However gamma rays treated plants showed significant gradual reduction in growth rate than the EMS treated plants. Based on probit analysis, LD50 (Lethal dose) value for gamma radiation and EMS have been fixed as 2.81 kR and 0.89% respectively.


Keywords: Hibiscus cv. Red Single, Mutation, Gamma radiation, Sensitivity, Survival, LD50
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How to cite this article:

Naveena, N., S. Subramanian, M. Jawaharlal, K. Iyanar and Chandrasekhar, C. N. 2020. Determination of Mutagenic Sensitivity in Hibiscus rosa-sinensis L. (Cultivar Red Single) to Physical and Chemical Mutagens.Int.J.Curr.Microbiol.App.Sci. 9(10): 1756-1763. doi: https://doi.org/10.20546/ijcmas.2020.910.213