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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 1230-1238
DOI: https://doi.org/10.20546/ijcmas.2020.910.148


Incidence of Potyviruses on Two Wild Yams Species, Potential Varietal Improvement Plant Genetic Resources, Involved in Farmers’ Domestication Process in Togo
Kwasi Dzola Ayisah1*, Gbenonchi Mawussi2 and Larounga Tchaniley1
1Department of Plant Breeding and Crop Science,
2Laboratory of Research on Agroresources and Environmental Health, Ecole Supérieure d’Agronomie, Université de Lomé, B. P. 1515, Lomé, Togo
*Corresponding author
Abstract:

Yams grown in Togo are severely infected by Yam mosaic virus (YMV) and Yam mild mosaic virus (YMMV), genus Potyvirus. The present work aims to study the susceptibility, to potyviruses, of wild species Dioscorea praehensilis and Dioscorea abyssinica, which can be exploited for the search for resistant genotypes. Thus, 107 leaf samples of D. abyssinica and 72 of D. praehensilis collected in the Maritime, Plateaux, Centrale and Kara regions were analyzed by ACP-ELISA to detect Potyviruses and then by RT-PCR to detect YMV and YMMV, using primers YMV1 & YMV2 and YMV-CP-2F & YMV-UTR-1R, respectively. Following ACP-ELISA analyzes, in Plateaux region, 20.69% of samples of D. abyssinica and 2.78% of D. praehensilis were positive for potyviruses. In Kara and Central regions, 33.33% and 1.41% respectively of D. abyssinica samples were positive. RT-PCR analyzes did not reveal any positive samples for YMMV. However, a leaf sample of D. abyssinica in domestication and another of D. praehensilis taken from near a yam plot were positive for YMV. The two yam species, less sensitive to Potyviruses, YMV, YMMV, could be potential sources of resistance.


Keywords: Potyvirus, Yam mosaic virus, Yam mild mosaic virus, Dioscorea abyssinica, Dioscorea praehensilis, Incidence rate
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How to cite this article:

Kwasi Dzola Ayisah, Gbenonchi Mawussi and Larounga Tchaniley. 2020. Incidence of Potyviruses on Two Wild Yams Species, Potential Varietal Improvement Plant Genetic Resources, Involved in Farmers’ Domestication Process in Togo.Int.J.Curr.Microbiol.App.Sci. 9(10): 1230-1238. doi: https://doi.org/10.20546/ijcmas.2020.910.148