National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Yams grown in Togo are severely infected by Yam mosaic virus (YMV) and Yam mild mosaic virus (YMMV), genus Potyvirus. The present work aims to study the susceptibility, to potyviruses, of wild species Dioscorea praehensilis and Dioscorea abyssinica, which can be exploited for the search for resistant genotypes. Thus, 107 leaf samples of D. abyssinica and 72 of D. praehensilis collected in the Maritime, Plateaux, Centrale and Kara regions were analyzed by ACP-ELISA to detect Potyviruses and then by RT-PCR to detect YMV and YMMV, using primers YMV1 & YMV2 and YMV-CP-2F & YMV-UTR-1R, respectively. Following ACP-ELISA analyzes, in Plateaux region, 20.69% of samples of D. abyssinica and 2.78% of D. praehensilis were positive for potyviruses. In Kara and Central regions, 33.33% and 1.41% respectively of D. abyssinica samples were positive. RT-PCR analyzes did not reveal any positive samples for YMMV. However, a leaf sample of D. abyssinica in domestication and another of D. praehensilis taken from near a yam plot were positive for YMV. The two yam species, less sensitive to Potyviruses, YMV, YMMV, could be potential sources of resistance.
Citations |
 |
 |
 |
 |
