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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 1214-1217
DOI: https://doi.org/10.20546/ijcmas.2020.910.145


Evaluation of in vitro Antibacterial Activity of Phage Lysate against Microbial Pathogen Isolated from Septic Wounds
Sanjay Shukla*, A. Nayak, R. K. Sharma, P. C. Shukla and R. V. Singh
Department of Veterinary Microbiology, College of Veterinary Science & A.H., Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India
*Corresponding author
Abstract:

In present study, we had isolated the bacteriophages from sewage material of various livestock farms, NDVSU, Jabalpur Madhya Pradesh. The recovered bacteriophages subjected for the identification with electron microscopy. The isolated bacteriophages had shown icosahedral symmetry. Phage had hexagonal head with the size of 52.20 nm in diameter and long tail of 109 nm. They were classified as a member of the Myoviridae family under the order of Caudovirale. These recovered bacteriophages were subjected to spottest to know the antibacterial activity against bacterial pathogens. All the recovered phage lysate showed the antibacterial activity specifically against the Staphylococcus aureus isolated from chronic septic wounds. These isolates of bacteriophage had limited host range due to their nature to infect the specific host. There was few isolates (ØVS4 ØVS5 and ØVS9) of bacteriophages were showing the lytic activity against the other microbial organism like Bacillus spp and E. coli. ØVS4, ØVS5 and ØVS9 three isolates of bacteriophage had broad host range. In vitro antibacterial activity of phage lysates can be used as a potential therapy against multiple drug resistant pathogens in the current era of antibiotic resistance crisis.


Keywords: In vitro, S. aureus Antibacterial activity, Phage lysate, Antibiotic resistance
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How to cite this article:

Sanjay Shukla, A. Nayak, R. K. Sharma, P. C. Shukla and Singh, R. V. 2020. Evaluation of in vitro Antibacterial Activity of Phage Lysate against Microbial Pathogen Isolated from Septic Wounds.Int.J.Curr.Microbiol.App.Sci. 9(10): 1214-1217. doi: https://doi.org/10.20546/ijcmas.2020.910.145