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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 1698-1706
DOI: https://doi.org/10.20546/ijcmas.2020.910.205


Cultural, Morphological and Molecular Characterization of Fusarium verticillioides causing Maize Ear Rot under Punjab Condition 
Kiranjot Kaur1*, Jaspal Kaur2, Anita Puyam3 and Karmjit Singh4
1Department of Agriculture, Khalsa College Amritsar (143002), India
2Department of Plant Breeding, Punjab Agricultural University, Ludhiana, Punjab, India
3Department of Plant Pathology, Rani Lakshmi Bai Central Agricultural University, Jhansi (284003), India
4University College of Agriculture, Guru Kashi University, Talwandi Sabo (151302), India
*Corresponding author
Abstract:

Maize ear rot caused by F. verticillioides which is prevalent on maize under Punjab conditions. The symptoms appear as white-pink fungal growth and causes “starburst” pattern of white streaks on the kernels. Thirty Fusarium isolates were isolated from the infected maize plant from different agro-chemical zones of Punjab. They are characterized on the basis of morphological, cultural characters and growth rate on Potato dextrose agar (PDA). The isolates produced micro-conidial 4.0-33.0 µm x 2.4-3.3 µm on PDA and macrocondia of 37.0-55.0 µm x 4.0-4.2 µm on Carnation leaf agar (CLA). Formation of sporodochia was observed on the Carnation leaf agar (CLA). The production of chalmydospores was not observed in any of the isolates. Isolates were grouped into three categories based on colony color and conidial size. The micro-conidial chains production was also observed on water agar infused with 0.2 perent KCl. Genomic DNA was extracted and was subjected to Polymerase chain reaction (PCR) by using species specific primers (PRO 1 PRO2, VER 1 VER2, VERT 1 VERT 2 and SUB1 SUB2). Out of thirty 27 isolates were confirmed  as F. verticillioides as they gave single amplicon of 800 bp with primer pair VERT 1 & VERT 2 specific for F. verticillioides.


Keywords: Maize ear rot, F. verticillioides, Sporodochia , VERT 1 & VERT 2, Species specific primers
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How to cite this article:

Kiranjot Kaur, Jaspal Kaur, Anita Puyam and Karmjit Singh. 2020. Cultural, Morphological and Molecular Characterization of Fusarium verticillioides causing Maize Ear Rot under Punjab Condition.Int.J.Curr.Microbiol.App.Sci. 9(10): 1698-1706. doi: https://doi.org/10.20546/ijcmas.2020.910.205