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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 1302-1309
DOI: https://doi.org/10.20546/ijcmas.2020.910.157


Perform Cluster Analysis to Assess the Differences for Storage Seed Protein Profile in the Cowpea Mutants
Madhu Choudhary*, K. Ram Krishna and Rajwanti Saran
Department of Plant Breeding and Genetics, S.K.N. College of Agriculture, SKN Agricultural University, Jobner303329 (Jaipur), Rajasthan, India
*Corresponding author
Abstract:

An investigation was carried out on 38 mutants derived from EMS (0.5%) mutagenesis of two cowpea varieties RC-19 and RC-101 to determine the variation in their profile of seed storage protein subunits through sodium dodecyl sulphate - polyacryl amide gel electrophoresis (SDS-PAGE).The protein bands of the 40 genotypes (parents and mutants) could be placed in five distinct regions. The protein bands of region I, II and V were monomorphic and intensely stained and identified to have a MW between 97.4 kD to 43 kD. Only certain bands of region III and IV were polymorphic. The binary data generated from the polymorphic bands over the genotypes were used to compute Jaccard’s similarity coefficients using NTSYS-pc software. The similarity matrix thus prepared was used to construct a dendrogram by UPGMA. The dendrogram distributed the 40 genotypes in 11 clusters. About 50% mutants were in one cluster and their parents in other clusters. One mutant in each group assumed unique place i.e. mutant 30 (dd) of RC101 and mutant 37 (kk) of RC 19. Clustering seemed to be independent of the seed attributes studied.  The protein content of the mutants was invariably reduced as compared to their parents and ranged from 21-30.3 %. It was concluded that results of the studies may be useful in selection of mutants for hybridization program for possible improvement of the quality of seed storage proteins in cowpea.


Keywords: Cowpea mutants, Seed storage protein, Clustering, SDS-page
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How to cite this article:

MadhuChoudhary, K. Ram Krishna and Rajwanti Saran. 2020. Perform Cluster Analysis to Assess the Differences for Storage Seed Protein Profile in the Cowpea Mutants.Int.J.Curr.Microbiol.App.Sci. 9(10): 1302-1309. doi: https://doi.org/10.20546/ijcmas.2020.910.157