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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 943-950
DOI: https://doi.org/10.20546/ijcmas.2020.910.113


Evaluation of Extraction Methods for Progesterone Metabolite Determination in Buffalo Feces by Immunoassay
D. Gupta1, G. Singh1*, G. Kashyap2, M. C. Pathak1, P. Patel1, M. Sarkar1, V. S. Chouhan1 and M. R. Verma3
1Division of Physiology and Climatology,
2Division of Pathology,
3Division of Livestock Economics, Statistics and Information Technology, IVRI, Bareilly, UP, India
*Corresponding author
Abstract:

The present study was planned to develop the best fecal extraction method for progesterone metabolite assay which is based on the concentration of fecal progesterone metabolite (FPM) (5α-pregnan-3α-ol-20-one), obtained in a dried fecal sample of buffaloes. Four clinically healthy female buffaloes of the same age group 4 to 7 years were used for the collection of fecal samples maintained under isomanagerial conditions with an intensive system at the LPM Section of the IVRI institute. The extraction of FPM (5α-pregnan-3α-ol-20-one) was done by various methods using organic solvents like methanol, diethyl ether, ethanol, and 90% methanol and found that extraction with 90% methanol the concentration of FPM (5α-pregnan-3α-ol-20-one) (231.98ng/g), is comparatively higher than other methods and best for a dried fecal sample of buffaloes. In conclusion, the above results suggest that FPM (5α-pregnan-3α-ol-20-one) value using 90% methanol as a solvent in the dried fecal sample assay having high value as compared to other solvents extraction methods like methanol, absolute ethanol, and diethyl ether, and we reported best for a dried fecal sample of buffaloes and preserved for further estimation.


Keywords: Extraction, Feces, Progesterone, Metabolite
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How to cite this article:

Gupta, D., G. Singh, G. Kashyap, M. C. Pathak, P. Patel, M. Sarkar, V. S. Chouhan and Verma, M. R. 2020. Evaluation of Extraction Methods for Progesterone Metabolite Determination in Buffalo Feces by Immunoassay.Int.J.Curr.Microbiol.App.Sci. 9(10): 943-950. doi: https://doi.org/10.20546/ijcmas.2020.910.113