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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:10, October, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 833-840
DOI: https://doi.org/10.20546/ijcmas.2020.910.099


Functional Analysis of Chalcone Synthase (Chs) Gene in Indian Wheat Cultivars in Response to Peg-Induced Drought Stress
Megha Singh*, Vaishali and Lalit Kumar
Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut: 250010 College Of Applied Education and Health Sciences, Meerut-250001, India
*Corresponding author
Abstract:

PEG- based in-vitro screening for drought tolerance has been proven to be a suitable method to effectively screen large sets of germplasm with good accuracy. In response to stress, plants produce secondary metabolites that stimulate the induction of different biochemical alterations which include synthesis of preventive phenolic compounds. CHS acts as the front-end coordinator of flavonoid biosynthesis and its regulation. The expression of CHS gene is affected by biotic and abiotic factors as well as pathogen attack. The aim of this study was to analyse the expression profile of CHS in wheat genotype under imposed drought stress. For this purpose 20 wheat genotypes were treated with different concentration of PEG to imposed rought condition. The result shows very high expression value of CHS gene for genotype HD2733 and K9107 as compared to genotype DBW71 and PBW553 at every level of polyethylene glycol concentration under imposed drought stress condition.


Keywords: Chalcone synthase gene, Drought stress, Functional analysis, Polyethylene glycol, RNA extraction

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How to cite this article:

Megha Singh, Vaishali and Lalit Kumar. 2020. Functional Analysis of Chalcone Synthase (Chs) Gene in Indian Wheat Cultivars in Response to Peg-Induced Drought Stress.Int.J.Curr.Microbiol.App.Sci. 9(10): 833-840. doi: https://doi.org/10.20546/ijcmas.2020.910.099
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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