PEG- based in-vitro screening for drought tolerance has been proven to be a suitable method to effectively screen large sets of germplasm with good accuracy. In response to stress, plants produce secondary metabolites that stimulate the induction of different biochemical alterations which include synthesis of preventive phenolic compounds. CHS acts as the front-end coordinator of flavonoid biosynthesis and its regulation. The expression of CHS gene is affected by biotic and abiotic factors as well as pathogen attack. The aim of this study was to analyse the expression profile of CHS in wheat genotype under imposed drought stress. For this purpose 20 wheat genotypes were treated with different concentration of PEG to imposed rought condition. The result shows very high expression value of CHS gene for genotype HD2733 and K9107 as compared to genotype DBW71 and PBW553 at every level of polyethylene glycol concentration under imposed drought stress condition.

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