Dalpatsagar reservoir was severely infested with many aquatic macrophytes throughout the year since last decade and modified the structure of habitat and influenced the aquatic organism. An experiment was conducted in Dalpatsagar reservoir Jagdalpur, Chhattisgarh during February 2019 to February 2020 throughout the year under Randomized Complete Block Design (RCBD) with three replications comprising seven treatments viz. Glyphosate (41 SL), Paraquat (24 SL) and 2,4-D (amine salt 58% SL) at 2.00 and 1.00 litre/ha with each herbicides including Absolute control. The herbicides were mixed with sticker (Latron AG-98) and sprayed in three replications by power spray machine (1HP HTP MAK ASPEE) mounted on the boat. The pH was 6.77 to 6.10 at observation of 3 to 120 days but it gradually increased 7.92 at 300 days of observation. The pH was lowest 6.40 with Glyphosate @ 2.00 litre/ha, EC was increased 0.59 dSm-1 till 300 days and it was significantly higher (0.41 dSm-1) value was observed. The value of TDS was also gradually increased 237 mg/l at 300 days, but after spray of herbicide, the value was reduced 199 mg/l with application of Glyphosate @ 2.00 litre/ha. CO3 and HCO3 of Dalpatsagar were increased 57.38 mg/l and 319 mg/l in response to Glyphosate applied @ 2.00 litre/ha at 300 DAS. The reduction of weed dry weight (g/ m2) was maximum with Glyphosate @ 2.00 litre/ha followed by 2,4-D @ 2.00 litre/ha at 20 DAS. Weed control efficiency was significantly higher in Pistia stratiotes (81.27%) and Nelumba nucifera (81.26%) with glyphosate @ 2.00 litre/ha at 5 and 20 DAS. Mean Weed control efficiency was higher (63.80%) with glyphosate @ 2.00 litre/ha with higher cost of control Rs. 6684 per ha followed by 2,4-D @ 2.00 litre/ha. This can be proved that Glyphosate @ 2.00 litre/ha was much effective herbicides to control aquatic weed population followed by 2,4-D @ 2.00 litre/ha with Rs. 5747 per ha than other herbicides.

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