Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 569-580
DOI: https://doi.org/10.20546/ijcmas.2020.910.068


Effect of Different Varieties, Media and Storage Temperature on in vitro Pollen Germination in Mango (Mangifera indica L.)
D. N. Dhamsaniya*, D. K. Varu and D. R. Mehta
College of Horticulture, Junagadh Agricultural University, Junagadh, Gujarat, India
*Corresponding author
Abstract:

A study was conducted to investigate in vitro pollen germination of four mango varieties, viz. Kesar, Alphonso, Mallika and Dudhpendo. Pollen grains were stored in three different media (n-hexane, paraffin oil and without media) under four different storage temperature (-20⁰C, -4⁰C, 4⁰C and room temperature). The experiment was laid out in Completely Randomized Design (CRD) with factorial concept. Germination media having 10% sucrose + 100 ppm H₃BO₃ + 300 ppm Ca(NO₃)₂ + 200 ppm MgSO₄ + 100 ppm KNO₃ used for in vitro pollen germination for all the varieties. Variation due to different varieties, media and temperature was found significant. Among four varieties, maximum germination was recorded in Kesar during 7, 14, 21, 28, 35, 42 and 56 days of storage, whereas, in Mallika at 49 days of storage. In storage media, n-hexane found best for retaining more germination ability during all storage days. Room temperature storage of pollen showed no germination from 21 days of storage. Maximum pollen germination was recorded at -20⁰C followed by -4⁰C. On the basis of interaction effect of results obtained, it could be concluded that mango pollen grains of Kesar and Mallika could be stored successfully up to 56 days in n-hexane at -20⁰C.


Keywords: Mango; Pollen storage, In vitro pollen germination, Storage media, Storage temperature
Download this article as Download

How to cite this article:

Dhamsaniya, D. N., D. K. Varu and Mehta, D. R. 2020. Effect of Different Varieties, Media and Storage Temperature on In vitro Pollen Germination in Mango (Mangifera indica L.).Int.J.Curr.Microbiol.App.Sci. 9(10): 569-580. doi: https://doi.org/10.20546/ijcmas.2020.910.068