Nematodes are the most abundant metazoans and highly diverse playing significant role in nutrient recycling, successful bioagent of insect pest and most importantly yield loss to crop. The model nematode (Caenorhabditis elegans) was first nematode which genome sequenced in 1998 and given impetus for future endeavor to generate genomic information for other nematodes particularly for PPNs. Comparative genomics is one of the approaches to predict the genes shared with different species and also to unravel the genes unique and novel to a particular species. In the present study we have used a graph-based method by utilizing Orthovenn 2 tool for orthologous analysis to compare and annotate the orthologous cluster between the genome of Anguina tritici, Ditylenchus destructor and Meloidogyne incognita.

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