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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 649-661
DOI: https://doi.org/10.20546/ijcmas.2017.604.080


Multidrug Resistant Bacillus cereus in Fresh Vegetables: A Serious Burden to Public Health
Bhavish Sood1*, Param Pal Sahota1 and Mandeep Hunjan2
1Department of Microbiology, Punjab Agricultural University, Ludhiana-141004, India
2Department of Plant Pathology, Punjab Agricultural University, Ludhiana-141004, India
*Corresponding author
Abstract:

The frequent inhabitation of B. cereus in fresh vegetables can critically damage the public health and economics. Fresh vegetables from three sources; Village fields, Supermarkets and Street Vendors were examined for microbiological safety for raw consumption. Among the seven salad vegetables that are integral to daily diet intake were found with high Food MPN count of >100g-1 (42.8-100%), total coliforms >10ml-1 (75.6-98.5%), faecal coliforms >10ml-1 (59.6-91.2%). The highest mean count of B. cereus (4.35 log cfu g-1) was found in carrots sourced from Village fields). The ability of Bacillus isolates to lyse erythrocytes can directly be correlated with the expression of haemolytic enterotoxin. Antibiotic resistance of Bacillus cereus isolates to four or more antibiotics may bud a serious perturbation in treating associated illness. Effectiveness of aminoglycoside, fluoroquinone, tetracycline and glycopeptides during this study could be considered before empirical therapy against B. cereus infections. PCR analysis of nineteen B. cereus isolates showed the presence of haemolytic enterotoxin gene hblA (100%), hblC (73.6%) and hblD (89.4%) and that of non-haemolytic enterotoxin nheA (100%) and nheC (100%). All the isolates were devoid of nheB gene. Their toxigenicity can further increase the risk of illness due to raw consumption of vegetables for which important interventions should be implemented for decontamination.


Keywords: Antibiotic resistance, B. cereus, Enterotoxin, Fresh vegetables, Raw consumption
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How to cite this article:

Bhavish Sood, Param Pal Sahota and Mandeep Hunjan. 2017. Multidrug Resistant Bacillus cereus in Fresh Vegetables: A Serious Burden to Public Health.Int.J.Curr.Microbiol.App.Sci. 6(4): 649-661. doi: https://doi.org/10.20546/ijcmas.2017.604.080