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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 117-127
DOI: https://doi.org/10.20546/ijcmas.2020.910.016


Plant Growth Promoting and Antagonistic Activity of Rhizospheric Strains Isolated from Wilt Affected Rhizosphere of Lentil (Lens culinaris Medik.) Crop against Wilt Pathogen Fusarium oxysporum f.sp.lentis
Shalini Srivastava1*, Adesh kumar1, Kusum Sharma2, Praveen Tiwari1,Minakshi Tiwari1, Kirti Srivastava3 and Alok Srivastava3
1Department of Plant Molecular Biology & Genetic Engineering, Acharya Narendra Deva University of Agriculture & Technology, Kumarganj, Ayodhya -224229 (UP) India
2ICAR-Indian Institute of Pulses Research, Kalyanpur, Kanpur, 208024 (U.P), India
3National Bureau of Agriculturally Important Microorganisms Mau Nath Bhanjan -275103 (UP) India
*Corresponding author
Abstract:

Fusarium wilt is a soil-borne disease, which negatively affects the lentil crop widespread in India and affecting the lentil crop productivity. So the present study was conducted to evaluate the efficacy of bioagents against wilt pathogen Fusarium oxysporum f.sp.lentis of lentil crop through antagonistic activity. Rhizospheric strains were isolated from wilt affected lentil plant in different regions of Eastern UP like Faizabad, Mau, Varanasi, Ghazipur. A total of 20 bacterial strains were isolated and tested for antagonistic activity by co-culture method against pathogen (Fusarium oxysporum sp.lentis), out of which 8 strains (B1, B2, B3, B4, B5, B6, B7, B8) showed antagonistic activity against Fusarium oxysporum f.sp.lentis. Further study identified that all the 8 strains also showed PGPR activity such as phosphate solubilization, Zn solubilisation, siderophore production, HCN production and IAA production. Out of these 8 bacterial strains, three strains B4, B5 and B8 were found to be more effective against the same pathogen on the basis of mycillium growth as compare to B1,B2,B3,B6,B7 strains as well as shows greater PGPR activities such as PO4+ and Zn+ solubilzation ranges from 3 strain minimum to maximum B7, B1 and B8 28%, 40% and 60% as compare to B4, B6, B5, B3, B2 8.33%, 8.33.%, 20, 20, 28 % and B5, B8 and B7 6.6% ,4%, 4%  B3, B4, B2, B1, 2.66%, 0.5%, 2.5, 2.66, strain minimum to maximum and siderophore, only 3 strains B1, B8 and B5, 20%, 33% and 50%. On the basis of qualitative analysis, NH3 production was maximum B1, (+++), B5 (+++), B8 (++) as compare to B2 (+), B3 (+), B4 (++), B6 (++), B7 (++). HCN B8 (+++), B5 (+++), B1 (++) and IAA production B4 (++), B5 (+++), B8 (++) as compare to 5 strain B1 (+), B2 (+), B3 (++), B6 (+), B7 (+) respectively. Study concluded that these strains characterized as bio-control agents along with beneficial PGPR activities and are found to have more efficient activity to suppress the wilt pathogen fusarium oxsporum f. sp. lentis in lentil that would further used for plant growth promotion of lentil crop.


Keywords: Bio-agents, Pathogen, Lentil crop, Wilt disease, Antagonism
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How to cite this article:

Shalini Srivastava, Adesh kumar, Kusum Sharma, Praveen Tiwari, Minakshi Tiwari, Kirti Srivastava and Alok Srivastava. 2020. Plant Growth Promoting and Antagonistic Activity of Rhizospheric Strains Isolated from Wilt Affected Rhizosphere of Lentil (Lens culinaris Medik.) Crop against Wilt Pathogen Fusarium oxysporum f.sp.lentis.Int.J.Curr.Microbiol.App.Sci. 9(10): 117-127. doi: https://doi.org/10.20546/ijcmas.2020.910.016