National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The healthy, disease free, young and juvenile shoot explants were selected for the experiment. The explants were collected from the fields of Ishved biotech Pvt. Ltd., washed several time with tap water for 30 min in order to wash off the external dust/contaminants and then by using liquid biological detergent Bavistin (2% w/v). Again 70% ethanol is used for 1 minute, after that process 0.1% mercury chloride (HgCl2) for 5 min. and rinsed the explants three to four time to remove the traces of HgCl2 and ethanol the by sterile water. BA concentrations 0.0, 0.5, 1.0, 1.5, 2.0 mg/L. in combination with NAA 0.1mg/L were prepared by dissolving them in 100% sterile water as stock. Then, MS media (34.4gm/l), sucrose was dissolved in distilled water. The pH of the medium was adjusted between 5.6-5.8 by using either 0.1 N Hcl or 0.1 N NaOH with the help of a digital pH meter. The volume (1000 ml) was finally adjusted and required amount of agar added into the media. Agar in the medium was completely melted by gentle heating up to 90ºC and 40 ml of medium was poured into pre sterilized culture bottles. The media was autoclaved at 121ºC at 15 lbs/square inch pressure for 20 minutes and then allowed to cool to room temperature and stored in culture rooms until further use. After sterilization explants were trimmed to 1.5-2 cm and inoculated on MS media supplemented with different combination of BA with constant NAA inoculation and room temperature was maintained at 25±2ºC with 16 hour photoperiod. Biometric observation No. of Shoots, shoot length, No. of roots, root length were observed periodically.
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