National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
CPV-2 infection in dogs is recognized as one of the most common diseases especially in young puppies of less than a year age. Keeping in view of frequency of the occurrence of infection, sometimes even in vaccinated animals, the present study was taken up with the objective of standardizing the Real time PCR for easy and rapid diagnosis of the CPV-2 virus. In the present investigation a total of 100 faecal samples/rectal swabs were collected from dogs suffering with gastrointestinal disorders and showing the symptoms of canine parvovirus, like bloody diarrhoea, vomiting and pyrexia. The samples were tested initially with SYBR green dye based real time PCR with different combinations of the cycles and the results obtained were further confirmed by conventional PCR. We could able to standardize the real time PCR which was more helpful in screening of the faecal samples further in large numbers.
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