National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was carried out to isolate Bacillus cereus from meat collected from Bikaner. In this study 50 raw poultry meat samples were collected from various retail meat outlets of Bikaner city. Out of 50 raw poultry meat samples, 14 (28%) samples were found positive for Bacillus cereus by culture and biochemical tests. The isolates showing positive reaction for the Catalase test, Voges Proskauer’s test, Arginine test, Nitrate reduction test, Citrate utilization test and sugar tests like Glucose, Sucrose, Arabinose and Trehalose while negative with ONPG test, Mannitol and Malonate test were confirmed as Bacillus cereus. Phenotypically identified B. cereus isolates were further analysed by polymerase chain reaction technique. The 14 isolates were subjected to PCR. Out of 14, 12 (85.71%) isolates possessed gyrB gene and were confirmed as B. cereus. Hence it was concluded that the occurrence of B. Cereus in foods especially in raw meat as a meat contaminant and causes food poisoning which is a serious public health concern and need to be addressed.
Citations |
 |
 |
 |
 |
