National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Tick infestation is a major reason for economic loss in livestock production in the tropical countries. However, animals show variation in resistance and susceptibility to tick infestation. Bos indicus breeds were reported to be more resistant to tick infestation than Bos taurus cattle. The present study aimed to identify polymorphisms in four quantitative trait loci (QTLs) that were stated to be associated with resistance/susceptibility to tick infestation, in a population comprising native Vechur breed (n=45) and crossbred cattle (n=74) of the Kerala Veterinary and Animal Sciences University farms. The QTLs were selected from the Animal QTL Database (https://www.animalgenome.org/cgi-bin/QTLdb/index). The single nucleotide polymorphisms (SNP) and the QTLs (in parenthesis) used in this study were: rs41661020 (#101148), rs43708490 (#135798), rs41577070 (#135800) and rs29009970 (#135801). Blood samples were collected from the animals, isolated the DNA and used for genotyping and polymorphism study by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and confirmed by sequencing. The results revealed polymorphic pattern for the SNPs rs41577070, rs41664020, and SNP rs43708490 in both Vechur and cross-bred populations. The SNP rs29009970 was monomorphic in the crossbred cattle, whereas, dimorphic in Vechur. Genotyping was done by direct counting and the allelic and genotypic frequencies were estimated by POPGENE 1.32. Chi-square test revealed that crossbreds and Vechur were in Hardy-Weinberg equilibrium for SNPs rs41577070, rs41664020, and rs43708490, whereas, the SNP rs29009970 showed a significant departure from the equilibrium.
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