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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 3078-3087
DOI: https://doi.org/10.20546/ijcmas.2020.909.380


Analysis of Single Nucleotide Polymorphisms in the QTLS Associated with Tick Resistance/Susceptibility in the Native Vechur and Cross red Cattle of Kerala State
Bhagyashree Kamble Mukhund1, C. N. Dinesh1*, P. M. Rojan, Reghu Ravindran2,A. Bindu3, V. N. Muhasin Asaf2, P. R. Sreeshma2 and T. Deepna2
1Department of Animal Genetics and Breeding, 2Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, India
3Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Mannuthy, Kerala Veterinary and Animal Sciences University, India
*Corresponding author
Abstract:

Tick infestation is a major reason for economic loss in livestock production in the tropical countries. However, animals show variation in resistance and susceptibility to tick infestation. Bos indicus breeds were reported to be more resistant to tick infestation than Bos taurus cattle. The present study aimed to identify polymorphisms in four quantitative trait loci (QTLs) that were stated to be associated with resistance/susceptibility to tick infestation, in a population comprising native Vechur breed (n=45) and crossbred cattle (n=74) of the Kerala Veterinary and Animal Sciences University farms. The QTLs were selected from the Animal QTL Database (https://www.animalgenome.org/cgi-bin/QTLdb/index). The single nucleotide polymorphisms (SNP) and the QTLs (in parenthesis) used in this study were: rs41661020 (#101148), rs43708490 (#135798), rs41577070 (#135800) and rs29009970 (#135801). Blood samples were collected from the animals, isolated the DNA and used for genotyping and polymorphism study by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and confirmed by sequencing. The results revealed polymorphic pattern for the SNPs rs41577070, rs41664020, and SNP rs43708490 in both Vechur and cross-bred populations. The SNP rs29009970 was monomorphic in the crossbred cattle, whereas, dimorphic in Vechur. Genotyping was done by direct counting and the allelic and genotypic frequencies were estimated by POPGENE 1.32. Chi-square test revealed that crossbreds and Vechur were in Hardy-Weinberg equilibrium for SNPs rs41577070, rs41664020, and rs43708490, whereas, the SNP rs29009970 showed a significant departure from the equilibrium.


Keywords: Ticks, tick resistance, QTL, SNP, Vechur, crossbred cattle, Kerala
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How to cite this article:

Bhagyashree Kamble Mukhund, C. N. Dinesh, P. M. Rojan, Reghu Ravindran, K. A. Bindu, V. N. Muhasin Asaf, P. R. Sreeshma and Deepna, T. 2020. Analysis of Single Nucleotide Polymorphisms in the QTLS Associated with Tick Resistance/Susceptibility in the Native Vechur and Cross red Cattle of Kerala State.Int.J.Curr.Microbiol.App.Sci. 9(9): 3078-3087. doi: https://doi.org/10.20546/ijcmas.2020.909.380