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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 431-440
DOI: https://doi.org/10.20546/ijcmas.2017.604.049


Prevalence of Extended-Spectrum β-Lactamases in Uropathogenic Klebsiella pneumoniae and Characterization of the bla Genes in a Tertiary Care Centre
Indu Menon*, Molly Madan and Ashish Asthana
Department of Microbiology, Subharti Medical College Meerut Uttar Pradesh, India
*Corresponding author
Abstract:

Urinary tract Infections caused by extended-spectrum β-lactamase (ESBL), producing Klebsiella pneumoniae are on a rise all over the world with high morbidity and mortality. This study was carried out to determine the presence of TEM, SHV and CTX-M genes in extended-spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae. A total of 300 Klebsiella pneumoniae isolates were collected and identified using traditional culturing and biochemical tests. Antibiotic susceptibility testing was performed by disc-diffusion method according to the CLSI guideline. Isolates were screened for ESBL and confirmed by phenotypic confirmatory disc diffusion test (PCDDT). 100 randomly selected isolates tested for the presence of ESBL encoding genes using PCR with specific primers for the detection of CTX-M, SHV and TEM genes using a standard protocol. Imipenem showed the highest antibacterial activity against ESBL producing K. pneumoniae. Based on the results of PCR, the prevalence of TEM, SHV and CTX-M genes among ESBLs-positive isolates was 74%, 27%, and 44% respectively. In conclusion, the rate of ESBL-producing K. pneumoniae was high in the present study. The bacterial resistance to many classes of antibiotic leads to limited treatment options. Since the management of infections caused by these organisms is difficult, it is important to control such strains in order to prevent and reduce their spread.
 


Keywords: Rice, BPH, Screening, Nilaparvata lugens.
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How to cite this article:

Indu Menon, Molly Madan and Ashish Asthana. 2017. Prevalence of Extended-Spectrum β-Lactamases in Uropathogenic Klebsiella pneumoniae and Characterization of the bla Genes in a Tertiary Care Centre.Int.J.Curr.Microbiol.App.Sci. 6(4): 431-440. doi: https://doi.org/10.20546/ijcmas.2017.604.049