|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A study was undertaken to investigate virulence gene profiles of shiga toxin-producing E. coli serogroups in diarrhoeic buffalo calves of Andhra Pradesh (AP) and Telangana States (TS). A total of 375 faecal samples from diarrhoeic buffalo calves of 1 to 90 days were collected from various districts in AP and TS, of which 302 E. coli (80.53%) were isolated. The virulence genes of STEC were detected using multiplex PCR and serogrouping was carried at National Salmonella and Escherichia centre, Central research institute, Kasauli, Himachal Pradesh. Among the virulence genes of STEC the stx1, stx2& hlyA genes carrying isolates constitutes about 4.63% of which 36% were identified as O157, distributed in Krishna and West Godavari districts of Andhra Pradesh. The serogrops of STEC associated with calf diarrhoeia includes O8, O9, O10, O26, O29, O49, O84, O86, O120, O121, O125, O126, O128, O141 and O157. The present study concluded that diarrhoeic buffalo calves are the source of about 15 STEC serogroups including O157 and O26 which are having public health significance.