Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 2696-2703
DOI: https://doi.org/10.20546/ijcmas.2020.909.336


Virulence Gene Profile and Serogroups of STEC in Diarrhoeic Buffalo Calves
M. Srivani1*, Y. Narasimha Reddy2, K.V. Subramanyam3, M. Lakshman4, T. Srinivasa Rao5 and K.L. Kavitha1
1Department of Veterinary Microbiology, NTR College of Veterinary Science,
Gannavaram, Krishna District, Andhra Pradesh, India
2Department of Veterinary Microbiology,
4Roska lab, College of Veterinary Science, Rajendranagar, Rangareddy District, Telangana State, India
3Department of Veterinary Microbiology, College of Veterinary Science, Proddatur, Andhra Pradesh, India
5Department of Veterinary Public Health, NTR College of Veterinary Science, Gannavaram, Krishna District, Andhra Pradesh, India
*Corresponding author
Abstract:

A study was undertaken to investigate virulence gene profiles of shiga toxin-producing E. coli serogroups in diarrhoeic buffalo calves of Andhra Pradesh (AP) and Telangana States (TS). A total of 375 faecal samples from diarrhoeic buffalo calves of 1 to 90 days were collected from various districts in AP and TS, of which 302 E. coli (80.53%) were isolated. The virulence genes of STEC were detected using multiplex PCR and serogrouping was carried at National Salmonella and Escherichia centre, Central research institute, Kasauli, Himachal Pradesh. Among the virulence genes of STEC the stx1, stx2& hlyA genes carrying isolates constitutes about 4.63% of which 36% were identified as O157, distributed in Krishna and West Godavari districts of Andhra Pradesh. The serogrops of STEC associated with calf diarrhoeia includes O8, O9, O10, O26, O29, O49, O84, O86, O120, O121, O125, O126, O128, O141 and O157. The present study concluded that diarrhoeic buffalo calves are the source of about 15 STEC serogroups including O157 and O26 which are having public health significance.


Keywords: Epidemiology, Diarrhoiec Buffalo calves, STEC, Serogroups
Download this article as Download

How to cite this article:

Srivani, M., Y. Narasimha Reddy, K.V. Subramanyam, M. Lakshman, T. Srinivasa Rao and Kavitha, K.L. 2020. Virulence Gene Profile and Serogroups of STEC in Diarrhoeic Buffalo Calves.Int.J.Curr.Microbiol.App.Sci. 9(9): 2696-2703. doi: https://doi.org/10.20546/ijcmas.2020.909.336