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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 2555-2565
DOI: https://doi.org/10.20546/ijcmas.2020.909.320


Study of Androgenesis in (Musa balbisiana) cv. Bhimkol Banana
and in vitro Regeneration of Haploids Using Isolated Microspore Culture
Manab Bikash Gogoi1*, Indrani Chetia1, Bidyut Kumar Sarmah1
Tankeswar Nath1, Swapnil Bhowal3, Kuntal Dey2 and Priyadarshini Bhorali2
1DBT-AAU Centre, Assam Agricultural University, Jorhat-785013, India
2Department of Agricultural Biotechnology, AAU, Jorhat-785013, India
3Indian Institute of Science Education and Research, Kolkata-700064, India
*Corresponding author
Abstract:

In-vitro androgenic haploids in Musa. balbisiana L cv. bhimkol were produced by anther culture at uninucleate stage of pollen. Young 30 day’s old immature male flowers with 5 stamens obtained by cutting out the bracts in between 20th to 30th were used as explants for induction of callus. The best medium for inducing callus from anthers were found to be MS (Murashige and Skoog) basal medium supplemented with 6-BAP (1.0 mgL-1) and IAA (Indole-3- acetic acid 2 mg/l.) with a frequency of 27.33±2.52 while best media for regeneration of shoots from callus was found to be MS medium supplemented with 7mg/l 6-BAP, 0.5mg/l IAA, 350 mg/l CH. Regenerated shoots were isolated and inoculated on MS medium supplemented with four different concentrations (0.25, 0.5, 1.0 and 2.0 mg/l) of IBA to induce root. IBA at a concentration of 1.0 mg/l produced best rooting. In our study out of 350 anthers inoculated only 12 plants were regenerated from anther callus and embryogenesis, all the plants were transferred and established in the potting mixture containing soil, vermicompost and farm yard manure in the ratio of 1:1:2. Using direct method of chromosome counting 58.33 % plants were found to be haploids and 41.66 % plants were diploids. This is the first report on the production of haploid plants in cv. bhimkol However, diploids banana plants were also found.


Keywords: Musa sp. cv. Bhimkol, Androgenesis, Indirect organogenesis, 2,4-Dichlorophenoxyacetic acid, 6-Benzlyaminopurine, Indole acetic acid, Casein hydrolysate
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How to cite this article:

Manab Bikash Gogoi, Indrani Chetia, Bidyut Kumar Sarmah, Tankeswar Nath, Swapnil Bhowal, Kuntal Dey and Priyadarshini Bhorali. 2020. Study of Androgenesis in (Musa balbisiana) cv. Bhimkol Banana and In-vitro Regeneration of Haploids Using Isolated Microspore Culture.Int.J.Curr.Microbiol.App.Sci. 9(9): 2555-2565. doi: https://doi.org/10.20546/ijcmas.2020.909.320