National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Clinical cases presented to Veterinary College Hospital, Bangalore, Karnataka were used as subjects for the study. Samples were collected from lesions of dogs showing clinical signs typical of pyoderma and were subjected to bacterial isolation and culture with mannitol salt agar, coagulase and catalase testing and biochemical characterization for species differentiation as per standard procedures. Of the 91 samples examined, 88 were coagulase positive staphylococci, which following biochemical characterization were classified as S.aureus (16 isolates) and S intermedius (72 isolates). These were further subjected to sensitivity testing for Methicillin / Oxacillin by disc diffusion as per standard procedures. Fourteen of the 88 isolates were found to be methicillin resistant of which 5 isolates were S aureus and 9 were S intermedius. Six of the methicillin resistant isolates (Four S intermedius and two S aureus as per biochemical characterization) were further subjected to DNA extraction and PCR targeting the mecA gene specific for methicillin resistance. Four of the 6 isolates (3 S intermedius and one S aureus) yielded 304 bp corresponding to mecA gene, one of the primary genes responsible for methicillin resistance The variation between the results observed by disc diffusion and PCR in this study may be due to the fact that there are additional genetic material responsible for methicillin resistance including genes encoding production of Panton Valentine Leucocidin (PVL) Thus the negative results of 2 of the isolates for mecA gene in the present study could be attributed to either overestimation of methicillin resistance by disc diffusion or it could be due to some other gene mediating resistance.
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