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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 2047-2055
DOI: https://doi.org/10.20546/ijcmas.2020.909.255


Prevalence and Characterization of Escherichia coli from Poultry Meat in Bhubaneswar
Iswar Anand Senapati1*, Rajashree Mishra1, Akshaya Kumar Kundu2,
Bidyut Prava Mishra3 and Prasana Kumar Rath4
1Department of Veterinary Microbiology, 2Department of Veterinary Physiology, 3Department of Livestock Products Technology, 4Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, OUAT, Bhubaneswar, India
*Corresponding author
Abstract:

E. coli are normal inhabitants of the gastrointestinal tract of animals, birds and humans of which only some strains have become highly adapted to cause diarrhoea and a range of extra-intestinal diseases. In the present study, a total of 320 raw poultry meat samples were collected from retail poultry meat market of Bhubaneswar, Odisha, India. A total of 224 E. coli isolates were obtained. The triturated samples were first inoculated on MLA and then on EMB agar, the colonies with greenish metallic sheen were considered as E. coli which was further confirmed by biochemical tests. The overall prevalence of E. coli observed during the present study was 70 per cent in the samples. In vitro antibiotic resistance patterns against 18 antibiotics revealed highest resistance to Oxytetracycline and highest sensitivity was exhibited to Tobramycin by all types of isolates. All the 224 E. coli isolates were subjected to biofilm production by Microtiter Plate Method and Congo Red Agar method. 68.75% of E. coli isolates were found to be positive for biofilm formation by both MTP method and CRA method. By MTP method 12.5%, 79.17% and 8.33% isolates are high, moderate and weakly positive respectively. By CRA method, 20.83%, 50% and 29.17% isolates are high, moderate and weakly positive respectively.


Keywords: E. coli, Chicken meat, Antibiotics, Biofilm
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How to cite this article:

Iswar Anand Senapati, Rajashree Mishra, Akshaya Kumar Kundu, Bidyut Prava Mishra and Prasana Kumar Rath. 2020. Prevalence and Characterization of Escherichia coli from Poultry Meat in Bhubaneswar.Int.J.Curr.Microbiol.App.Sci. 9(9): 2047-2055. doi: https://doi.org/10.20546/ijcmas.2020.909.255