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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 341-346
DOI: https://doi.org/10.20546/ijcmas.2017.604.037


Biochemical, Physiological and Morphological Characterization of the Bacterial Isolate causing Stalk Rot of Sorghum as Erwinia chrysanthemi
Prachi Singh*, Yogendra Singh and Jyotika Purohit
Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar, Pin-263145, U.S. Nagar, Uttarakhand, India
*Corresponding author
Abstract:

Stalk rot caused by Erwinia chrysanthemi is one of the most destructive diseases of sorghum crop. The bacterium was isolated from infected sorghum plants collected from livestock research centre, G.B. Pant University of Agriculture and Technology, Pantnagar, India. Biochemical, physiological and morphological characterization of the isolate was done for confirmation of the bacterium species. Bacterial suspension [0.7% Tween-40 (v/v) + 1 × 107 cell/ml] of isolate was inoculated in leaf whorl with the help of atomizer for pathogenicity testing. Biochemical and physiological analysis confirmed the taxonomic assignation of E. chrysanthemi as the causal organism of stalk rot of sorghum. Growth assay on Erwinia specific medium, crystal violet sodium polypectate (CVP) medium, the specific medium for E. chrysanthemi at 37oC, expressing characteristic deep pit, provided the confirmatory result. The typical stalk rot disease symptoms on sorghum plant, further validated the authenticity of the test microorganism.


Keywords: Characterization, CPV medium, Erwinia chrysanthemi, Sorghum, Stalk rot.
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How to cite this article:

Prachi Singh, Yogendra Singh and Jyotika Purohit. 2017. Biochemical, Physiological and Morphological Characterization of the Bacterial Isolate causing Stalk Rot of Sorghum as Erwinia chrysanthemi.Int.J.Curr.Microbiol.App.Sci. 6(4): 341-346. doi: https://doi.org/10.20546/ijcmas.2017.604.037