National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Stalk rot caused by Erwinia chrysanthemi is one of the most destructive diseases of sorghum crop. The bacterium was isolated from infected sorghum plants collected from livestock research centre, G.B. Pant University of Agriculture and Technology, Pantnagar, India. Biochemical, physiological and morphological characterization of the isolate was done for confirmation of the bacterium species. Bacterial suspension [0.7% Tween-40 (v/v) + 1 × 107 cell/ml] of isolate was inoculated in leaf whorl with the help of atomizer for pathogenicity testing. Biochemical and physiological analysis confirmed the taxonomic assignation of E. chrysanthemi as the causal organism of stalk rot of sorghum. Growth assay on Erwinia specific medium, crystal violet sodium polypectate (CVP) medium, the specific medium for E. chrysanthemi at 37oC, expressing characteristic deep pit, provided the confirmatory result. The typical stalk rot disease symptoms on sorghum plant, further validated the authenticity of the test microorganism.
Citations |
 |
 |
 |
 |
