National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was done at the Frozen Semen Laboratory, U.T. of Jammu & Kashmir, Hukkal, Jammu and Division of V.G.O., F.V. Sc & A.H., SKUAST- J, R.S. Pura, during the period between December 2017 and May 2018. This investigation was carried out as a pioneer study with the objective to study the effect of natural antioxidant Aloe vera as a semen additive on cryopreservation of semen. Semen samples (n=10) from mature cattle bull stationed at Frozen semen laboratory, U.T. of Jammu & Kashmir, Hukkal, Jammu, were used to evaluate the effect of Aloe vera additive at post-dilution and at post-thaw stage. The semen sample was extended with Tris-Egg-Yolk-Citric-acid-Fructose-Glycerol (TEYCAFG) extender and were split into two groups: Group 1: TEYCAFG without any additive/ control and group 2: TEYCAFG + Aloe vera (5µl/ml). Progressive motility, live spermatozoa, acrosomal integrity, sperm abnormality, hypo-osmotic swelling test (HOST) was evaluated at both post-dilution and post-thaw stage, whereas, oxidative stress tests viz. malondialdehyde (MDA), catalase (CAT) and superoxide dismutase (SOD) were evaluated at only post-thawed stage. Group 2 i.e. Aloe vera group, showed significant (p<0.05) increased progressive motility, live spermatozoa, acrosomal integrity and HOST positive spermatozoa, while significant (p<0.05) decreased sperm abnormalities in post-thawed semen. In oxidative stress evaluation the MDA level was significantly (p<0.05) decreased, whereas, CAT level significantly (p<0.05) increased in group 2 in comparison to control group. It was concluded that addition of Aloe vera (5µl/ml) as semen additive improves semen quality and minimize oxidative stress to the spermatozoa during cryopreservation of semen.
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