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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:9, September, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 549-561
DOI: https://doi.org/10.20546/ijcmas.2020.909.070


Morphological and Molecular Characterization of Complete Panicle Emergence Mutant Lines for Assessing Genetic Relatedness
Suneel1, G. S. Laha1, A. P. Padmakumari1, R. M. Sundaram1, V. Subba Rao1, P. Sudhakar2, A. Krishna Satya2 and M. S. Madhav1*
1ICAR-Indian Institute of Rice Research, Hyderabad, India
2Department of Biotechnology, Acharya Nagarjuna University, Guntur, India
*Corresponding author
Abstract:

Morphological characterization of thirteen promising CPE (complete panicle emergence) mutants of Samba Mahsuri was done using thirty four agro-morphological traits by following DUS (Distinctiveness, Uniformity and Stability) guide lines. There was no variation observed in twenty two characters while in 12 characters there was variation among the individual mutants. Among all the traits, Culm attitude showed higher variation but there was no variation in the panicle emergence. Genetic relatedness among the mutants was assessed by the hyper polymorphic SSR markers. Correlation of phenotypic and genotypic data among mutants revealed that three mutants (CPE-1, CPE-9 & CPE-10) showed high genetic similarity with wild type (95%, 98.33% & 96.66%) as well as negligible variation in tested morphological characters. Whereas two mutants (CPE-12 & 13) showed more variation   in morphological characters as well as less genetic similarity (86.66 & 86.66 %) with wild type was observed. But all the genotypes used in this study exhibits complete panicle emergence, which is differ from the wild type parent. This detailed characterization of Samba Mahsuri CPE mutants is very important for rice breeding from the standpoint of selection and conservation of different mutants for further utilization in crop improvement programmes and also to seek protection under PPV&FR Act, 2001 of India.


Keywords: Samba Mahsuri, Complete panicle emergence, DUS, morphological traits, Mutants, Correlation, Variation, PPV & FRA Act

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How to cite this article:

Suneel, B., G. S. Laha, A. P. Padmakumari, R. M. Sundaram, L. V. Subba Rao, P. Sudhakar, A. Krishna Satya and Madhav, M. S. 2020. Morphological and Molecular Characterization of Complete Panicle Emergence Mutant Lines for Assessing Genetic Relatedness.Int.J.Curr.Microbiol.App.Sci. 9(9): 549-561. doi: https://doi.org/10.20546/ijcmas.2020.909.070
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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